支气管哮喘患儿体内嗜酸性粒细胞比例与CTLA-4基因型的关系

Relationship between eosinophilic rat and the polymorphisms of CTLA-4 in infants with bronchial asthma

目的探讨支气管哮喘患儿体内嗜酸性粒细胞比例与细胞毒T淋巴细胞相关抗原4(CTLA-4)第一外显子+49(A/G)、启动子-318(C/T)基因多态性之间的关系。方法采用聚合酶链反应-限制性片段多态'性(PCR-PFLP)技术分析哮喘患儿26例及对照组30例+49(A/G)、-318(C/T)基因多态性;采用血细胞分析仪及瑞氏染色检测外周血及痰中嗜酸性粒细胞数。结果与正常儿童相比,哮喘患儿外周血及痰中嗜酸性粒细胞比例明显增高;基因型为GG型患儿外周血及痰中嗜酸性粒细胞比例都明显高于AG型及AA型,而在启动子-318(C/T)及对照组各基因型内无差异。结论嗜酸性粒细胞在哮喘的发生中起着重要作用且与CTLA-4第一外显子+49(A/G)基因型有关。

Objective To explore the relationship between the rate of eosinophils in peripheral blood or in sputum and the cytotoxic T lymphocyte associated antigen 4 （ CTLA -4） gene exon 1 ＋ 49 （A/G） and promoter - 318 （C/G） genetic polymorphisms in asthmatic children. Methods The genotypes at position exonl ＋ 49 （A/G） and promoter -318 （C/G） of CTLA -4 were determined by polymerase chain reaction - restriction fragment length polymorphism （PCR - RFLP） in asthmatic and healthy children. Eosinophils were counted by hematology analyzer and Wright staining. Results Compared with the control group, the number of the eosinophils in asthmatic children＇s peripheral blood was larger, and that in GG genotype was higher than in AG and AA. But there was no difference in -318（C/T） and control group. Conclusion Eosinophils play an important role in asthmatic pathogenesis, related to genotype of CTLA -4 gene exon 1 ＋ 49（A/G）.