乳酸杆菌对过敏性鼻炎大鼠血清IgE、IL-4和IL-5含量的影响

Effect of latobacillus gasseri on serum IgE,IL-4 and IL-5 in rats with rhinitis

目的:探讨乳酸杆菌对过敏性鼻炎(AR)大鼠血清中IgE、IL-4和IL-5含量的影响。方法:SD大鼠,40只随机分为正常组、模型组、乳酸杆菌组及阳性药对照组4组,每组10只。乳酸杆菌组每天用乳酸杆菌活菌制剂1.5mg/g以无菌稀释液溶解后灌胃3周;正常组、模型组及阳性药对照组均予无菌稀释液灌胃3周。模型组、乳酸杆菌组及阳性药对照组建立大鼠卵白蛋白致敏模型。阳性药对照组灌胃氯雷他定5mg/kg,连续10d。采用ELISA法定量检测试验各组大鼠血清IgE、IL-4、IL-5的含量。结果:模型组IgE、IL-4、IL-5的含量显著高于正常组(P<0.05);乳酸杆菌组IgE、IL-4、IL-5的含量均显著低于模型组(P<0.05);乳酸杆菌组各项指标与阳性药对照组比较差异无统计学意义(P>0.05)。结论:乳酸杆菌可降低AR大鼠血清中IgE、IL-4和IL-5含量,减轻鼻黏膜变应性炎症。

Objective： To explore the effect of lactobacillus on serum IgE,IL-4 and IL-5 levels of allergic rhinitis（AR） rats.Methods： 40 rats SD were randomly divided into normal group,model group,lactobacillus group and positive drug control group.Lactic acid bacteria group was given stomach irrigation of lactobacillus living bacterium preparations,1.5mg/g diluted by sterile solution everyday for 3 weeks;normal group,model group and positive drug control group were given diluted sterile solution for 3 weeks.Rat LuanBai protein sensitization model was established.Positive drug control group was irrigated with loratadine,5 mg/kg,for 10d;ELISA quantitative test was used to determine serum IgE,IL-4,IL-5 content.Results： IgE,IL-4,IL-5 content in model group was significantly higher than that in normal group（P〈0.05）;IgE,IL-4,IL-5 content in lactic acid bacteria group was significantly lower than that in model group（P〈0.05）;the index of lactobacillus group compared with that of positive drug group had no statistical significance.Conclusion： Lactic acid bacteria can reduce IgE,IL-4 and IL-5 content in AR rat＇s serum,and reduce nasal mucosa allergic inflammation.