下呼吸道164株念珠菌鉴定和药敏结果分析

Identification of lower respiratory tract candida and analysis of drug sensitivity

目的:分析住院病人下呼吸道念珠菌的感染分布和药敏情况,为临床用药提供依据,方法:痰标本按要求接种血平板后,挑取可疑菌落经涂片,革兰氏染色证实为念珠菌后转种于念珠菌显色平板,根据菌落色彩进行鉴定,对鉴定出的念珠菌进行两性霉素B、5-氟胞嘧啶、伊曲康唑、氟康唑4种抗真菌药敏试验。结果:送检下呼吸道分泌物标本的住院病人下呼吸道念珠菌阳性率为24.7%,以白色念珠菌为主,占71.3%。念珠菌对4种抗真菌药物的敏感率依次为:两性霉素B94.5%、5-氟胞嘧啶92.1%、氟康唑70.1%、伊曲康唑34.1%。结论:临床应合理应用抗生素,降低念珠菌的感染率,加强念珠菌的检测和药敏分析,以两性霉素B和5-氟胞嘧啶抗真菌敏感性较好。

Objective：To identify infection distribution and drug sensitivity of lower respiratory tract candida of hospitalized patients,and provide theoretic basis for clinic medication.Methods： Doubtful colony was selected and smeared on plate after innoculating sputum specimens on the blood agar plate based on requirement,and then smeared on the Candida Chromogenic Medium if it was confirmed by Gram staining based on colony color.The antifungal susceptibility test of identified candida was conducted with Amphotericin B,5-Fluorocytosine,Itraconazole,and Fluconazole.Results： The lower respiratory candida infection rate of clinical patients was 24.7%,candida albicans was in majority（71.3%）.The sensitivity rates of candida to 4 above-mentioned drugs were： Amphotericin B（94.5%）,5-Fluorocytosine（92.1%）,Fluconazole（70.1%）,and Itraconazole（34.1%）.Conclusion： Antibiotics should be applied reasonably to reduce the infection rate of candida,and the antifungal sensitivity test should be strengthened（the antifungal sensitivity of Amphotericin B and 5-Fluorocytosine are better）.