新生儿巨细胞病毒感染的胞外体二肽基肽酶Ⅳ及血清中白细胞介素-6,-8水平的研究

Levels of Dipeptidyl Peptidase Ⅳ and Interleukin -6,-8 in Neonates of Cytomegalovirus Infection

目的探讨巨细胞病毒(CMV)感染对新生儿免疫功能的影响及胞外体(EXO)二肽基肽酶Ⅳ(DPPⅣ,CD26)及血清白细胞介素(IL)-6,-8水平在CMV感染发生发展过程中的作用。方法选择2008年1月至2010年12月山东大学齐鲁医院儿科收治的53例CMV感染新生儿为研究对象,纳入CMV组,选择同期入院的42例非CMV感染新生儿(CMVIgM呈阴性,血、尿中CMV-DNA<103拷贝/mL)纳入对照组。采用ELASA法检测EXO-DPPⅣ活性及血清IL-6,-8水平,并进行相关统计学分析(本研究遵循的程序符合山东大学齐鲁医院人体试验委员会制定的伦理学标准,得到该委员会批准,分组征得受试对象监护人的知情同意,并与其签署临床研究知情同意书)。两组新生儿的性别、年龄及胎龄等比较,差异无统计学意义(P>0.05)。结果 CMV组新生儿EXO-DPPⅣ活性及血清IL-6,-8水平均较对照组显著升高,且差异均有统计学意义(P<0.05)。结论 CMV感染后,体内免疫平衡被打破,出现酶类及细胞因子分泌异常。EXO-DPPⅣ活性及IL-6,-8水平与炎症反应密切相关。

Objective To study the levels of dipeptidyl peptidase Ⅳ （DPPⅣ）of exosome（EXO） and interleukin（IL）-6,-8 in serum of neonates with cytomegalovirus（CMV） infection,to investigate the influence of CMV on neonates′ immune function, and the roles of DPPⅣ,and IL-6,-8 in serum which played in the process of CMV infection. Methods From January 2008 to December 2010,a total of 53 neonates with CMV infection were collected as CMV group,meanwhile the other 52 non-CMV infection neonates were recruited as control group.By ELASA method to detect the levels of EXO-DPPⅣ and IL-6,-8 in serum,then analysis the differences between two groups. Informed consent was obtained from the parents of each participating neonate.There had no significant difference between two groups among gender,age,gestational age etc.（P0.05）. Results The levels of EXO-DPPⅣ, and IL-6,-8 in serum in CMV group were much higher than those in control group, and had significance difference （P0.05）. Conclusions CMV can break normal immune balance,leading abnormality of enzymes and cytokine. There is close correlation between inflammatory reaction,DPPⅣ,and IL-6,-8.