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液相芯片技术在检测肺癌患者血清VEGF中的应用

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摘要 目的探讨用液相芯片技术检测肺癌患者及健康人血清中血管内皮生长因子(VEGF)的表达水平。方法用LuminexFLEXMAP3D仪和Human Cytokine/Chemokine试剂盒检测血清VEGF的表达水平,并确立其最适条件,对120例肺癌患者和348例健康人血清VEGF水平进行检测,分析两组对象血清VEGF水平的差异。结果确立了液态芯片技术检测血清VEGF的最适条件。肺癌患者血清VEGF均值为(295±205)pg/mL,明显高于健康人的(160±154)pg/mL,差异具有统计学意义(t=9.119,P<0.01)。结论确定的液态芯片技术检测血清VEGF的最适条件,且肺癌患者血清VEGF表达水平显著高于健康人。
出处 《临床检验杂志》 CAS CSCD 北大核心 2012年第12期969-970,共2页 Chinese Journal of Clinical Laboratory Science
基金 江苏省恶性肿瘤多学科联合诊治技术服务中心项目(BM2011118) 南京工业大学2012年大学生创新创业训练计划项目
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