1L-8和MMP-9在非小细胞肺癌患者组织及血清中表达水平的研究

Overexpression of IL-8 and MMP-9 Confer High Malignant Phenotype in Patients with Non-small Cell Lung Cancer

背景与目的白细胞介素一8（interleukin一8，IL一8）曾被认为是一种具有趋化作用的炎症因子，近年的研究认为它和基质金属蛋白酶一9（matrixmetalloproteinase一9，MMP一9）均为与肿瘤生长和转移密切相关的细胞因子，本研究通过检测IL-8、MMP一9在非小细胞肺癌患者组织和血清中的表达，研究二者与临床病理特征之间的联系，分析肺癌组织和血清中IL-8、MMP-9表达的相关性，并探讨IL-8、MMP-9作为肿瘤标志物评估非小细胞肺癌患者病情进展程度的效能。方法应用酶联免疫吸附实验（enzyme-linkedimmunosorbentassay，ELISA）检测141例非小细胞肺癌患者、40例健康人和40例肺良性疾病患者血清中IL-8、MMP-9的水平。采用免疫组化sP法检测95例非小细胞肺癌、21例良性疾病肺组织及25例正常肺组织中IL-8和MMP-9的表达。结果IL-8、MMP-9在非小细胞肺癌患者血清和组织的表达水平明显高于肺良性疾病对照组和健康对照组，其差异有统计学意义，且随着临床病理分期的升高而升高。在肺癌患者组织和血清中，IL-8与淋巴转移均有较强的相关性，在肺癌组织中IL-8和MMP-9的表达具有很强的相关性（r=O．765）。结论IL18、MMP-9表达水平与非小细胞肺癌的进展密切相关，特别是IL-8与肺癌的淋巴转移具有明显的相关性，其机制可能是通过上调MMP-9实现的。

Background and objective IL-8 （interleukin-8） has been identified as a chemotactic factor,but recent found that IL-8 and matrix metalloproteinase-9 （MMP-9） are important cytokines which are closely related to the growth and metastasis of tumor.The aim of this study is to explore the relationship between IL-8,MMP-9 expressions and clinical pathological features of non-small cell lung cancer （NSCLC） patients and evaluate the diagnostic potential of IL-8,MMP-9 as tumor markers.Methods The serum levels of IL-8 and MMP-9 were detected by enzyme-linked immunosorbentassay （ELISA） in 141 NSCLC patients,40 healthy adults and 40 patients with benign pulmonary disease.The expressions of IL-8 and MMP-9 were detected by immunohistochemical method iN95 NSCLC tissues,and 21 benign disease lung tissues,25 normal lung tissues as control. Results The level of expression of IL-8 and MMP-9 in serum and tissue of NSCLC was significantly higher than that of healthy and benign respiratory disease,and the expression was gradually increased with the upgrade of clinicopathological stage.The serum and tissue expression of IL-8 and MMP- 9 in NSCLC patients with lymph node metastasis was remarkably higher than that without lymph node metastasis.There is an positive correlation （r=0.765） between IL-8 and MMP-9 in the tissue of NSCLC patients.Conclusion This study has confirmed that IL-8,MMP-9 expressions are related to the development of NSCLC.There is an obvious correlation between IL-8 expression and lymph node metastasis,IL-8 may facilitate the lymph node metastasis by up-regulating MMP-9 expression.Serum level of IL-8 is a valuable auxiliary parameter in diagnosing lymph node metastases of NSCLC with good sensitivity and specificity.