中国北方地区汉族人群MCP-1基因多态性与肺癌相关性研究

Relationship between Genetic Polymorphism of MCP-1 and Lung Cancer in Han Nationality of North China

目的探讨中国北方地区汉族人群单核细胞趋化因子蛋白-1（monocytechemoattractantprotein1，MCP-1）基因-2518位点多态性与肺癌的相关性。方法应用聚合酶链反应一限制性片段长度多态性（polymerasechainreaction-restrictionfragmentlengthpolymorphism，PCR-RFLP）的方法对中国北方地区汉族人群134例肺癌患者和82例正常对照进行MCP-1基因-2518位点基因多态性检测。结果AA基因型和GG基因型频率在肺癌病人和对照组间差异有统计学意义。AA基因型患肺癌的相对风险度增加（OR=2．645，x2=6．532，P=-0．011），GG基因型患肺癌的相对风险度降低（OR=0．519，x2=4．929，P=-0．026）。肺癌病人中非小细胞肺癌患者AA基因型和GG基因型频率在病人和对照组间差异有统计学意义，AA基因型患病的相对风险度增加（OR-3．138，x2=8．905，P=0．003），GG基因型患病的相对风险度降低（OR=0．516，x2=4．613，P=0．032）。小细胞肺癌患者各基因型频率与对照组差异无统计学意义。结论中国北方地区汉族人群MCP-1基因-2518位点多态性与非小细胞肺癌相关，与小胞肺癌不相关。

Objective To study the relationship between monocyte chemoattractant protein 1 （ MCP-1 ） -2518A/G polymorphism and lung cancer in Han nationality of North China. Methods One hundred and thirty-four unrelated consecutive patients with lung cancer （ 112 with NSCLC, 22 with SCLC ） and 82 healthy individuals were studied. The polymorphisms of MCP-1 -2518A/G were detected with polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） . Results The distribution of AA, AG and GG genotypes of MCP-1 -2518 was significantly different in lung cancer patients compared with controls （ x 2 =8.486, P = 0.014） . There was a significant increase in the frequency of the AA genotype （OR = 2.645, x 2 ~ 6.532, P = 0.011 ） and a significant decrease in the frequency of the GG genotype （ OR = 0.519, x 2 = 4.929, P = 0.026） in the lung cancer patients, compared with controls. In the NSCLC patients the frequency of the AA genotype was higher than controls （ OR - 3.138, x 2 =8.905, P = 0.003 ） and the frequency of the GG genotype was lower than controls （ OR = 0.516, x 2 = 4.613, P = 0.032） . The frequencies ofAA, AG andGG genotypes in SCLC patients and controls had no difference. Conclusion This preliminary study showed that MCP-1 -2518A/G polymorphism was associated with NSCLC but not SCLC.