摘要
以通蓖5号品种的蓖麻花药为试验材料，研究了不同浓度的蔗糖、不同生长调节剂及pH值对愈伤组织诱导的影响结果表明，蔗糖在20—40g／L之间均有较高的愈伤诱导率，且随蔗糖浓度的增加而升高；当ZT为3．0mg／L、NAA为1．0mg／L愈伤组织诱导最高；当pH值在6．0时，愈伤组织诱导效果最佳，诱导率达到最高为27．95％，且此时褐化率较低．
Ricinus communis ＇tong bi 5＇ as experimental material, we studied the effect of sugar concentration, various of plant growth regulators and pH values on the anthers for callus induction, the higher callus induction rate was detected with sugar concentration between 20g/L and 40g/L and increased with the sugar concentration; Combined with ZT3.0mg/L and NAA1.0mg/L has highest induction frequency of pollen callus; when the pH values was 6.0, the callus induction rate can be reach 27.95%,and the lower callus browning rate.
出处
《内蒙古民族大学学报（自然科学版）》
2012年第6期670-673,共4页
Journal of Inner Mongolia Minzu University：Natural Sciences
基金
国家自然科学基金(31060194)
内蒙古民族大学科研项目(NMD1128)
关键词
蓖麻花药
愈伤组织
诱导率
Ricinus communis
Anther
Callus
Induction rate
