壳寡糖促神经元生长的作用

Effect of chitooligosaccharides on the neurite outgrowth of neurons

目的研究壳寡糖(COSs)对大鼠神经元生长的影响。方法以体外培养的背根神经节(DRG)和DRG神经元为研究模型,通过神经丝蛋白-H(NF-H)免疫荧光细胞化学染色和Leica QWin软件检测不同浓度壳寡糖(0.0、0.1、0.2 mg/ml)作用5 d对DRG和DRG神经元突起生长的影响;通过Western blot检测不同浓度壳寡糖(0.05、0.1、0.2 mg/ml)作用DRG神经元12 h后,对DRG神经元中NF-H和生长相关蛋白-43(GAP-43)表达的影响。结果与对照组相比,中、高剂量壳寡糖(0.1、0.2 mg/ml)显著促进DRG突起的生长(均P<0.01);0.2 mg/ml剂量的壳寡糖明显促进DRG神经元突起的生长(P<0.05)。与对照组相比,体外培养的DRG神经元加入壳寡糖作用12 h后,0.2 mg/ml剂量壳寡糖组的NF-H和GAP-43表达量明显增加(分别P<0.05和<0.01)。结论壳寡糖可有促进体外培养的DRG和DRG神经元突起的生长,并可促进NF-H、GAP-43的表达。

Objective To determine the effect of chitooligosaccharides （COSs） on the neurite outgrowth neuron of rats in vitro. Methods Dorsal root ganglion （DRG） explant and dissociated DRG neuron were cul- tured, and neurofilament-H （NF-H） fluorescent immunocytochemistry and Leica QWin software was performed to detect the neurite outgrowth of cultured DRG and DRG neuron treated with COSs （0.05, 0.1 and 0.2 mg/ ml）. Western blot was used to analyze the expression changes of NF-H and growth associated protein-43 （GAP-43） 12 h after treatment with COSs （0.05, 0. 1 and 0.2 mg/ml） in the dissociated DRG neuron. Re- suits The result of NF-H fluorescent immunocytochemistry showed that compared with the control group COSs （0. 1 mg/ral and 0, 2 mg/ml） promoted the neurite outgrowth of cultured DRG （P 〈0.01）. The effect of dif- ferent concentrations of COSs on neurite outgrowth of DRG neuron was similar to the effect on DRG. Compared with the control group, 0.2 mg/ml COSs promoted the neurite outgrowth of cultured DRG neuron （P 〈0.05）. The results of Western blot showed that compared with control group, the protein levels of NF-H and GAP43 were up-regulated in disscciated DRG neurons after treated with O. 2 mg/ml COSs treatment for 12 h （P 〈0.05 and P 〈0.01 ）. Conclusion COSs could promote the neurite outgrowth of cultured DRG and DRG neuron, and increase the expression of NF-H and GAP43.