非小细胞肺癌组织和患者外周血中CD40的表达及其临床意义

Expression of CD40 in NSCLC tissue and peripheral blood and its clinical significance

目的探讨非小细胞肺癌(NSCLC)组织和患者外周血中CD40的表达及其临床意义。方法采用免疫组织化学和酶联免疫吸附试验(ELISA)等方法检测经胸外科手术切除的60例NSCLC组织、15例癌旁肺组织和患者外周血中CD40的表达情况。结果 (1)NSCLC组织中CD40阳性表达率较癌旁肺组织明显增加(P<0.05);(2)有淋巴结转移组的CD40阳性表达率明显高于无淋巴结转移组(P<0.05)。Ⅲ～Ⅳ期NSCLC组织CD40阳性表达率明显高于Ⅰ～Ⅱ期(P<0.05)。(3)NSCLC外周血清可溶性CD40(sCD40)的表达水平明显高于健康对照组(P<0.05);(4)NSCLC患者外周血清sCD40含量和NSCLC组织中CD40分子表达成正相关关系(P<0.05)。结论 NSCLC患者体内CD40含量增加可增强肺癌的侵袭和免疫逃逸能力;患者外周血清sCD40含量升高可能和肺癌细胞表面CD40分子释放有关。

Objective To explore the relationship of CD40 expression in non-small cell lung cancer （NSCLC） and its clinical significance. Methods Immuno-histochemical staining （IHS） and enzyme- linked immunosorbent assay （ELISA） were performed to detect expression of CD40 in 60 NSCLC tissues, 15 paratumor tissues and their peripheral blood. Results （ 1 ） CD40 positive tumor cells could be detec- ted in NSCLC tissues（ P 〈 0.05 ）. （2） Expression rate of CD40 positive tumor cells in tumor tissues with lymph node metastases was higher than those without lymph node metastases （P 〈 0.05 ）. CD40 expres- sion in tumor tissues in stage Ⅲ～Ⅳ was higher than those in stage Ⅰ-Ⅱ. （P 〈 0.05）. （3） Level of sol- uble CD40 （sCD40） of NSCLC group was higher than the health control group. Further analysis demon- strated levels of sCD40 in serum was positively correlated with expression of CD40 in NSCLC tissues. Conclusion There is relationship between CD40 expression in NSCLC and clinical stage and metastases. CD40 may participate in tumor progression of disease and play an important role of tumor immune escape through enhancing the ability of invasion.