脾脏及Tuftsin在亚硝酸盐诱发胃癌过程中的作用

EFFECTS OF SPLEEN AND TUFTSIN ON GASTRIC CARCINOGENSIS INDUCED BY MNNG ( N -METHYL -N' -NITROSO-N-NITROGUANIDINE)

本文通过实验性胃癌模型的建立,观察脾脏及Tuftsin的免疫功能对肿瘤发生的影响。 大鼠随机分为切脾组、切脾后注射Tuftsin组、模拟切脾组。术后第8周起各组大鼠连续自由饮取t50mg/L浓度的MNNG溶液共32周,同时对一组切脾动物开始注射Tultsin。结果提示脾脏在肿瘤免疫中有双重作用。脾脏具有一定的防止瘤前病变及抗早期肿瘤的作用;对于进展期肿瘤,脾脏介入了肿瘤引起的机体免疫抑制状态的形成过程,有促肿瘤生长作用。脾脏特异的因子Tuftsin,可增加组织局部炎症反应从而发挥其抗肿瘤的作用,同时也提示胃粘膜慢性炎症与胃癌发生并无直接联系。作者提出了在脾外伤和胃癌手术时作保留脾脏手术的具体意见,并认为Tuftsin如能用于临床,将是肿瘤生物学治疗的一个新的途径。

Experimental gastric carcinogenesis was induced in order to investigate the effects of immunologic function of the spleen and Tuftsin on gastric carcinogenesis.A total of 180 Wistar rats were randomized into 3 groups : Group A-spenectomy performed, Group B-subcutaneous injection of Tuftsin after splenectomy and group C-laparotomy without splenectomy. Eight weeks after surgical procedures, all the groups were given 150μg/ml MNNG in tap water ad libitum while group B was given Tuftsin ( 5μg, twice a week ) for 32 weeks.It is suggested that the spleen can avoid precancerous pathologic changes and exert, to a certain extent, an antitumor function in carci-nogenesis or in early cancer. With the development of cancer, the spleen can be involved in the formation of host immunosuppression initiated by cancer itself and subsequently promote the cancer growth. Tuftsin is a specific antitumor fartor of the spleen. Our results suggest that local inflammation caused by tuftsin is one of the approaches to antitumor effects that tuftsin possoses, and that there is no direct relation between chronic gastritis ard geresis. The cli-nical use of tuftsin will be a new approach to Biological oncotherapy.