occludin在人宫颈癌中的表达及其临床意义

Expression and clinical significance of occludin in human cervical cancer

目的:探讨紧密连接蛋白occludin的表达与人宫颈癌发生、发展、转移和预后的关系。方法:原位杂交检测occludinmRNA的表达;免疫组织化学和Western blot检测occludin的表达,并结合44例患者的临床资料进行分析。结果:occludinmRNA的表达在宫颈癌的大体类型和分化程度间差异均有统计学意义(P<0.05);occludin的表达在宫颈癌分化程度间差异有统计学意义(P<0.05);宫颈癌旁组织的occludin表达水平高于宫颈癌组织(P<0.05);宫颈癌组织与宫颈癌旁组织及正常宫颈组织的occludin表达水平差异均有统计学意义(P<0.01);随访满5年和满3年存活患者的occludin mRNA和满3年存活患者的occludin表达水平均高于病死患者(P<0.05)。结论:occludin在人宫颈癌组织中低表达,其表达与宫颈癌分化程度及患者生存期皆有一定关系,可作为宫颈癌诊断和预后判断的新指标。

Objective：To investigate the relationship between the expression of occludin and the occurrence,development,metastasis and prognosis of human cervical cancer. Methods：The mRNA and protein expression of occludin were detected by in situ hybridization,immunohistochemistry and Western blotting,which combined with 44 patients clinical data were analyzed. Results：The occludin expression of different cervical cancer type and histologic differentiation was statistical different（P0.05）.The expression of occludin the adjacent tissues of cervical cancer tissue was higher than that of tumor tissues（P0.05）.The occludin expression levels in the adjacent tissues of cervical cancer tissue,tumor tissue and normal tissue were statistical different（P0.01）.The occludin mRNA expression levels of the survival patients with following up for 3 to 5 years were higher than that of the death patients（P0.05）. Conclusions：The expression of occludin is low in human cervical cancer,which has a positive correlation to the histologic differentiation of cervical cancer and survival time of patients and can be a new indicator for cervical cancer diagnosis and prognosis.