反式-BPDE诱发人胎气管上皮细胞p53基因突变的研究

STUDY ON THE POINT MUTATION OF THE p53 TUMOR SUPPRESSOR GENE IN HUMAN FETAL TRACHEAL EPITHELIUM CELL TREATED WITH TRANS-BPDE

目的 :探讨苯并 (a)芘在人肺癌发生中的作用。方法 :用苯并 (a)芘的代谢产物反式 -BPDE ,作用于体外培养的人胎气管上皮细胞 8～ 1 2周后 ,用PCR -SSCP方法检测 p53抑癌基因第 6,7,8外显子的点突变情况。 结果 :经反式 -BPDE处理的人胎气管上皮细胞 ,p53第 7,8外显子有点突变 ,而这些细胞都未出现典型的转化细胞形态特征的改变。结论 :反式 -BPDE可诱发人胎气管上皮细胞 p53基因点突变 ,此抑癌基因的点突变早于细胞形态学的改变。

Purpose: In order to study the effects of trans-BPDE on the development in human lung cancer, human fetal tracheal epithelium cells (HFTEs) cultured in vitro were exposed to trans-BPDE, a metabolite of benzo(a)pyrene\. Methods: For 8～12 weeks, the point mutations in exon 6～8 of p53 tumor suppressor gene in these HFTEs were detected by PCR-SSCP assay. Results: The results showed that the HFTEs treated with trans-BPDE had point mutations in exons 7 and 8 of p53 tumor suppressor gene, even though no malignantly transformed characteristics were observed in cellular morphology. Conclusion: these results suggest that trans-BPDE can induce point mutation in p53 tumor suppressor gene in these HFTEs and the point mutation and activation of p53 may occur in the cells before morphological transformation.