低剂量光动力诱导活性氧产生治疗视网膜母细胞瘤

Low Power Photodynamic Therapy for Retinoblastoma Mediated by Reactive Oxidative Damage

视网膜母细胞瘤(Retinoblastoma,RB)是一种最常见的儿童眼癌,传统的化疗和放疗可能会诱发二次肿瘤的产生。光动力疗法(photodynamic therapy,PDT)作为一种具有选择性的非入侵疗法在肿瘤治疗中展现了很好的应用前景。本课题通过实验观察了不同浓度焦脱镁叶绿酸-a(PPa)光动力作用对人视网膜母细胞瘤细胞株Y79细胞ROS产率和相应诱导凋亡能力的影响。结果表明,伴随逐步上升的PPa浓度(0.2、0.4、0.6μmol/L),Y79细胞的ROS产量和相对的凋亡率都明显上升,初步证明了PPa光动力杀伤视网膜母细胞瘤的可行性。

Retinoblastoma （RB） is the most common primary intraocular malignancy of infancy, traditional chemother- apy or brachytherapy may induce secondary sarcoma. Photodynarnic therapy （PDT） , a non-invasive selective therapy may be preferable. In this study, we observed the photodynamic effect of different dosages of PPa on Y79 cells through detecting the ROS production and corresponding cell apoptosis. The results indicated that the ROS production and corre- sponding cell apoptosis ratio increased obviously with an escalating PPa dosage （0.2,0.4, 0.6 μmol/L） , it is a primary proof of the feasibility of PPa-based photodynamic therapy for retinoblastoma cells killing.