摘要
In order to study the eco-physiological mechanisms of C. korshinskii adaptation to extreme drought stress, we investigated the variations of water content in soil, leaves, and stems, the chlorophyll a and b and the carotenoid content in leaves and stems, as well as changes of chloroplast ultrastructure in 2-year-old C. korshinskii specimens during a progressive soil drought process （by ceasing watering until all leaves were shed） and a subsequent rehydmtion process. During the dehydration process, the chlorophyll a and b and carotenoid contents in the leaves decreased, as did the carotenoid content in the stems. During the 4-day rehydration process, the chlorophyll a and b and carotenoid contents in the leaves and stems increased and gradually returned to normal levels. During ongoing drought stress, chloroplasts in the leaves broke away from cell walls and appeared in the center of cells. Under severe drought stress, the mesophyll ultrastmcture and chloroplast configuration in leaves were irreversibly disturbed, as mani- fested by the inner and outer membranes being destroyed; the thylakoid system disintegrated, the starch grain disappeared, and parts of cell tissue were dismantled into debris. However, the mesophyll ultrasWacture and chloroplast configuration in the stems remained complete. This indicates that C. korshinskii utilizes leaf abscission to reduce the surface area to avoid damage from ex- treme drought stress, and maintains chloroplast integrity and a considerable amount of chlorophyll to enable a rapid recovery of photosynthesis under the rehydration process.
In order to study the eco-physiological mechanisms of C. korshinskii adaptation to extreme drought stress, we investigated the variations of water content in soil, leaves, and stems, the chlorophyll a and b and the carotenoid content in leaves and stems, as well as changes of chloroplast ultrastructure in 2-year-old C. korshinskii specimens during a progressive soil drought process （by ceasing watering until all leaves were shed） and a subsequent rehydmtion process. During the dehydration process, the chlorophyll a and b and carotenoid contents in the leaves decreased, as did the carotenoid content in the stems. During the 4-day rehydration process, the chlorophyll a and b and carotenoid contents in the leaves and stems increased and gradually returned to normal levels. During ongoing drought stress, chloroplasts in the leaves broke away from cell walls and appeared in the center of cells. Under severe drought stress, the mesophyll ultrastmcture and chloroplast configuration in leaves were irreversibly disturbed, as mani- fested by the inner and outer membranes being destroyed; the thylakoid system disintegrated, the starch grain disappeared, and parts of cell tissue were dismantled into debris. However, the mesophyll ultrasWacture and chloroplast configuration in the stems remained complete. This indicates that C. korshinskii utilizes leaf abscission to reduce the surface area to avoid damage from ex- treme drought stress, and maintains chloroplast integrity and a considerable amount of chlorophyll to enable a rapid recovery of photosynthesis under the rehydration process.
基金
supported by the National Natural Sciences Foundation of China (Nos. 30900171, 91025026)
the China Postdoctoral Science Foundation (No. 20090450186)
