含饱和氢气肾保存液对大鼠肾脏冷缺血再灌注损伤的影响

Effects of hydrogen-rich kidney preservation solution on renal cold ischemia/reperfusion injury in rats

目的评价含饱和氢气肾保存液对大鼠肾脏冷缺血再灌注损伤的影响。方法健康雄性Wistar大鼠24只，周龄8～10周，体重200—250g，采用随机数字表法，将其随机分为3组（n=8）：对照组（H1组）大鼠仅切除右。肾；普通肾保存液组（H2组）大鼠采用冷缺血再灌注模型，用4℃普通HC-A肾保存液对左肾行冷灌注和冷保存；含饱和氢气肾保存液组（也组）大鼠操作同H2组，灌注液及保存液换用自制的4℃含饱和氢气HC—A肾保存液。于再灌注24h时抽取下腔静脉血样，测定血清BUN、Cr、TNF-α和IL-6浓度；切取左肾，测定肾组织MDA和8-羟基脱氧鸟苷（8-OndG）含量，光镜下观察肾组织病理学结果。结果与H1组相比，H2组和H3组大鼠血清BUN、Cr、TNF—α和IL-6浓度及肾组织MDA和8-OHdG含量均升高（P〈0．05）；与H2组相比，H3组血清BUN、Cr、TNF—α和IL-6浓度及肾组织MDA和8-OHdG的含量均降低（P〈0．05）。H1组。肾组织形态结构未见明显异常，H2组肾小管损伤明显，H3组肾小管损伤较H2组减轻。结论含饱和氢气肾保存液可明显减轻大鼠肾脏冷缺血再灌注损伤。

Objective To investigate the effects of hydrogen-rich HC-A solution, the self-made kidney preservation solution, on renal cold ischemia/reperfusion （I/R） injury in rats. Methods Twenty-four healthy male Wistar rats, aged 8-10 weeks, weighing 200-250 g, were randomly divided into 3 groups （ n = 8 each） ： control group （H1 group）, common kidney preservation solution group （HE group） and hydrogen-rich HC-A kidney preservation solution group （H3 group）. In H1 group, only the right kidney was removed. In H2 group, the left kidney was perfused with and cold stored in 4 ℃ common HC-A kidney preservation solution. In H3 group, the left kidney was perfused with and cold stored in a container filled with 4 ℃ common HC-A kidney preservation solution. Blood samples were obtained from the inferior vena cava at 24 h of reperfusion to detect the levels of serum blood urea nitrogen （BUN）, ereatinine （Cr）, TNF-α and IL-6. Left kidneys were removed for determination of malondialdehyde （MDA） and 8-hydroxy-2′-deoxyguanosine （8-OHdG） contents and for examination of the pathological changes in renal tissues （by light microscopy） .Results The levels of serum BUN, Cr, TNF-α and IL-6 and contents of MDA and 8-OHdG were significantly higher in H2 and H3 groups than in H1 group, and lower in 143 group than in H2 group （ P 〈 0.05）. There were no significant pathological changes in renal tissues in H1 group, the damage to renal tubules was obvious in HE group and the damage to renal tubules was significantly ameliorated in H3 group as compared with H2 group. Conclusion Hydrogen-rich kidney preservation solution can attenuate renal cold I/R in jury in rats.