基于FTIR的祖师麻有效部位化学成分整体分析与评价

Integrity Analysis and Assessment of Chemical Constituents of Zushima Effective Parts Based on FTIR

采用傅里叶变换红外光谱、二阶导数光谱、共有峰率和变异峰率的双指标序列等方法,首次对祖师麻中3种已知含量及功效的标准物质、不同提取方法提取的有效部位及渗漉液的不同萃取部位进行了结构特征的分析和鉴别。结果表明,祖师麻甲素及7-OH香豆素在1700cm^(-1)附近内酯及芳环在1600cm^(-1)附近有强吸收,紫丁香苷芳环v_C=c1589、1509cm^(-1)骨架振动及羟基v_(O-H)3563cm^(-1)的吸收峰较强。加热回流提取的有效部位在3300cm^(-1)处有较强的羟基V_(O-H)伸缩振动或氨基酸等v_(N-H)伸缩振动的叠加,渗漉法提取的有效部位在此处也有较强吸收峰,且3518cm^(-1)处的V_(N-H)较为明显。另外,两法提取的有效部位红外图谱共有峰率仅为45%,且变异峰率高达83.8%。其不同萃取部位的红外一维图谱均较好的体现了C=O、C—H、O—H、N—H及甲基、亚甲基的伸缩振动;对比各萃取部位的二阶导数谱,发现石油醚部除745—715cm^(-1)附近外,其余位置峰数明显少于其他3个萃取部位,乙酸乙酯部的峰数较多且峰形较好,氯仿和正丁醇部峰数与之相差不多,但峰形较弱,通过双指标法得出氯仿部和石油醚部所含物质种类相差极大,而氯仿部和乙酸乙酯部较为相近。

Fourier transform infrared spectroscopy,second derivative spectra,dual index sequence analysis of common and variant peak ratio were firstly used to analyze and identify the structure characteristics of 3 and standard substance,effective parts by different extraction method and different extraction of seeping fluid,contents and efficacy of which were known in Zushima.The results showed that lactone and aromatic ring of daphnetin and 7-hydroxycoumarin respectively had strong absorption in 1700cm^（-1） and 1600cm^（-1）,the absorption peaks of aromatic ring（υ_（c=c）1589,1509cm^（-1））and hydroxyl（vo-H3563cm^（-1））of syringin were strong.The effective parts were extracted by heating and refluxing had strong composition of hydroxy vo-h stretching vibration or amino acidυ_（N-H） stretching vibration in 3300cmυ,and effective parts were extracted by seeping fluid also had strong absorption peak in 3300cm^（-1）,υ_（N-H） was obvious in 3518cm＂1.Besides,common peak ratio of effective parts by two extraction methods was 45%,and variant peak ratio reached 83.8%.The IR 1D spectra of different extraction relatively reflected stretching vibration of C=O,C—H,O—H,N—H,methyl and methylene,compared with second derivative spectra of each extraction position,peak numbers of petroleum ether extraction were obvious less than 3 other extraction except 745—715cm^（-1） spectra range,peak numbers of ethyl acetate extraction were more with good spike,while chloroform extraction and n-butyl alcohol extraction were similar with weak spike.Chemical constituents of chloroform extraction and petroleum ether extraction were largely different by dual index sequence analysis,but chloroform extraction and ethyl acetate extraction were similar.