清除羟基自由基法评价水果抗氧化能力

Evaluation of Oxidation Resistance of Fruit by Eliminating Hydroxyl Radicals

在酸性介质中,Fenton体系能够产生羟基自由基并可迅速氧化四乙基罗丹明B,通过光度法检测其在550nm处测定吸光度的变化,可以间接的测定羟基自由基。水果中含有许多抗氧化成分可以消除溶液中的羟基自由基,从而使溶液的吸光度下降程度减弱,据此建立了一种测定水果对羟基自由基清除的_方法,实验对常见的4种水果的抗氧化活性进行了评价,以橙子的抗氧化活性最强。

Tetraethyl rhodamine R is oxidized in the presence of hydroxyl radicals that were produced by the Fenton system in acidic medium,determination of the change of absorbance at 550nm with photometry can indirectly determine hydroxyl radicals.Many antioxidant ingredients in fruits can remove hydroxyl radicals in solution,thus,absorbance of solution was weakened,a method was established to determine scavenging method of hydroxyl radicals by fruits.The experiment evaluated antioxidant activities of four fruits,and orange was the strongest.