琼胶分解菌Cellvibrio sp. BR的发酵条件及酶学性质研究

Study on the Fermentation Conditions and Agarase's Properties of Agar Degradation Bacteria Cellvibrio sp. BR

[目的]对琼胶分离菌Cellvibrio sp.BR的发酵条件及酶学性质进行研究。[方法]通过对琼胶分离菌Cellvibrio sp.BR发酵条件的优化,筛选该产酶菌株的最佳发酵条件,并对其酶学性质进行初步研究。[结果]该菌株产琼胶酶的最佳发酵条件为:在25℃、起始pH7.0的条件下培养36 h;该酶作用的最适pH为7.0～8.0,最适温度为35℃,最适底物质量分数为1.1%;该酶具有明显的底物特异性。[结论]为琼胶酶的进一步工业化生产提供了理论依据。

[Objective] The aim was to study the fermentation conditions of agar degradation bacteria Cellvibrio sp.BR and the enzymatic properties of its agarase.[Method] The optimal fermentation conditions of Cellvibrio sp.BR were screened by the optimization of its enzyme production conditions,and then the enzymatic properties of agarase were studied.[Result] The optimal fermentation conditions of Cellvibrio sp.BR were that incubated bacteria for 36 h at 25 ℃and pH 7.0.The optimal temperature,pH and agar substrate concentration for the agarase were 35 ℃,7.0-8.0 and 1.1%,respectively.And the agarase had significant substrate specificity.[Conclusion] It provided theoretical basis for the further industrial production of agarase.