不同生态型云南引种玛咖的多糖含量及多糖纯化工艺研究

Comparison of Polysaccharides Content in Different Eco-types of Maca Cultivated in Yunnan and the Purification Process of Polysaccharides in Maca

[目的]探讨不同生态型玛咖中的活性成分总多糖含量间的差异,并研究玛咖多糖的提取工艺。[方法]苯酚-硫酸法分别测定了云南丽江地区引种的4种生态型的玛咖块根中的多糖含量,并进行分析比较。玛咖多糖的基本纯化步骤为水提、醇沉、脱蛋白、脱色。[结果]依照颜色不同而划分为4种不同生态型的玛咖中总多糖含量有所不同,几种玛咖块根的总多糖含量顺序为黑色>紫色>绿色>黄色。多糖纯化的最优方法为80%乙醇沉淀、Sevag法脱蛋白。[结论]玛咖多糖的含量与其生态型有一定的关系,因此不同生态型的玛咖,其生理活性可能有一定的差别。

[Objective] To compare the polysaccharide content among four eco-types of cultivated Maca along with its purification technique.[Method] The content of polysaccharides in cultivated Maca in Yunnan was determined with Con-H2SO4-phenol spectrophotometry method.The purification of Maca polysaccharides was performed in the procedure of water-extraction,ethanol-precipitation,protein-removal and decoloration.[Result] The eco-types of Maca were classified by the color of the rhizomes,so the polysaccharides content detected in different eco-types was arrayed as： black purple green yellow.The optimized purification method of Maca polysaccharide was determined as follows： Extracting with 80% ethanol-water and removing protein with Sevag reagent.[Conclusion] The polysaccharide content in Maca has a certain relationship with its eco-types,Maca in different eco-type has differences in biological activity.