胃肠道间质瘤石蜡组织DNA倍体及CD117流式细胞术分析

Assessment of DNA ploidy and CD117 expression in paraffin imbedding gastrointestinal stromal tumor tissiues by flow cytometry

目的研究流式细胞术(FCM)检测DNA倍体和CD117对胃肠道间质瘤(GIST)的诊断价值。方法对52例GIST和10例食管平滑肌瘤组织的石蜡包埋块切片经过脱蜡水化,酶消化处理,制成单细胞悬液。同时,制备8例健康体检者外周血单细胞悬液,用FCM分别检测DNA倍体和CD117表达。结果 GIST瘤组织细胞出现异倍体阳性率23.1%(12/52),其中极低度/低度危险组未出现异倍体,中度/高度危险组出现12例,两组异倍体表达阳性率差异有统计学意义(P<0.05)。52例GIST瘤组织CD117均高表达,食管平滑肌瘤CD117弱表达,两组间CD117表达荧光强度差异有统计学意义(P<0.05)。GIST极低度/低度危险组CD117荧光强度与中度/高度危险组相比,CD117表达差异无统计学意义(P>0.05)。结论 DNA异倍体可提示GIST的中高危险度,FCM检测组织单细胞CD117表达可用于GIST的快速筛查。

Objective To investigate the prognostic significance of DNA ploidy and CD117 expression in gastrointestinal stromal tumors（GISTs）.Methods 52 cases of GIST wax tissue blocks were dewaxed in xylene and rehydrated in a sequence of decreasing concentrations of alcohol,digested by pepsin,and made into single cells suspension.Meanwhile the peripheral blood of 8 healthy subjects were made in to single cell suspension,then DNA ploidy and CD117 were determined by flow cytometry.Results DNA aneuploidy was seen in 12 cases（23.1%） and it was not seen in very low/low GIST group（14/0）,12 DNA aneuploidy were occurred in intermediate/high GIST group,there was significantly differennce between the two groups（P0.05）.52 GIST samples showed high CD117 expression,the parallel group still had weak CD117 expression.There was difference significantly between the two group（P0.05）;But there was no statistically difference between very low/low GIST group and intermediate/high GIST group（P0.05）.Conclusion our results suggest that DNA aneuploidy represents high risk group of GISTs.To detect the CD117 expression in tissue by using flow cytometry maybe workably for clinical GIST diagnostic screening.