NGF体外对精子活力的影响(英文)

Effect of NGF on the sperm motility of human in vitro

目的观察神经生长因子(NGF)体外对精子活力的影响,确定NGF的最佳量效和时效关系,探索NGF体外对精子活力的影响以及和男性不育的关系。方法分别用0.1、1、10μmol/L的NGF对精子活力进行研究,以探讨最佳浓度。将10μmol/LNGF加入精液中在1h内每隔10min用CASA检测精子的活力变化。结果 NGF可显著增加精子的平均速率、直线速率、曲线速率和线性系数,差异有统计学意义(P<0.01);增加鞭打频率差异有统计学意义(P<0.05)。1μmol/L的NGF和0.1μmol/L的NGF对精子活力的促进作用相比有统计学差异,10μmol/L浓度组和1μmol/L浓度组差异没有统计学意义。NGF反应30min后对精子活力的影响最大,一直保持到1h以后。结论外源性给予NGF可显著增加健康人和不育症患者a级运动精子比例,促进精子活力。

Objective Motility is an important physiological characteristic of a mature sperm. Nerve growth factor（NGF） is a protein essential for the development, maintenance and survival of the peripheral and central nervous systems. NGF and its receptors TrkA and p75 are widely expressed in the testis,accessory reproductive organ,and the epididymal sperms. In the present study, we investigated the role of NGF on human sperm motility. Methods Use 0.1,1 and 10μmol/L concentrations of NGF,on sperm mo- tility study to investigate the optimal concentration. Use CASA to detect Sperm motility changes every 10 minutes in an hour after 10 μmol/L NGF was added to the semen. Results The parameters of sperm motility increased after NGF incubation had significant difference, in particular, VAP, VSL, VCL, BCF and LIN mean were significantly increased more than 32 %. MAD, STR, ALH and WOB mean had no notable difference. Furthermore, NGF promotes the sperm motility in a time- and dose- dependent manner. In ad- dition,the enhancement of NGF on sperm motility was more stronger than those of sperm culture medium. Conclusion Our find- ings suggest that NGF plays a promoted role in human sperm motility.