血清铁自动化分析及其在肾病综合征中的临床意义

Automatic analysis of serum iron and its clinical significance in the nephrotic syndrome

目的评价亚铁嗪法检测血清铁,并探讨血清铁与肾病综合征(NS)的关系。方法用全自动生化分析仪采用亚铁嗪比色法检测血清铁,并检测100例NS患者的血肌酐、尿素、尿酸、尿β2-微球蛋白(β2-MG)浓度。结果不同浓度血清铁的检测结果线性相关,r2=0.9999,回收率平均值为97.14%,批内与批间重复性试验的CV值分别为0.94%、1.13%;检测灵敏度为1.25μmol/L。NS患者的血清铁降低无统计学意义(P>0.05),血清铁与尿素、尿酸、肌酐、β2-MG无相关性(P>0.05)。结论血清铁自动化分析快速、灵敏、准确、简便,符合临床需要。NS患者的血清铁浓度降低,降低程度与肾小球损伤程度无明显关系。

Objective To evaluate ferrozine method analysis serum iron, the association between serum iron and nephrotic syn- drome（NS） was studied. Methods Serum iron was measured with colorimetric method of ferrozine using Hitachi 7600-120E auto- matic biochemical analyzer, serum creatinine, urea, uric acid and β2-MG of nephrotic syndrome group（100 cases） were measured. Re- sults Measurement of different concentrations of serum iron had linear correlation, r2 = 0. 999 9. The average recovery rate was 97.14%. Within run and between run CV values were 0.94% and 1.13% respectively. The sensitivity was 1.25 μmol/L. The re- ducing of serum iron of patients with nephrotic syndrome was not significant（P〉0.05）, no significant linear correlations were found between serum iron and urea,uric acid,creatinine,β2-MG（P〉0.05）. Conclusion Automatic analysis of serum iron provides a rapid, sensitive,accurate and easy way,and it is suitable for clinical application. Serum iron concentrations of nephrotic syndrome patients is reduced,the degree of reducing has nothing to do with glomerular injury.