摘要
目的观察复方光明胶囊对实验性大鼠视网膜光损伤后Bax、bcl-2的影响.方法 SD大鼠30只(60只眼),随机分为高、中、低剂量组,模型组和正常组,每组6只(12只眼).造模前7 d给药,连续灌胃2周.除正常组以外各组实验用大鼠距角膜(150±3)mm照射,使大鼠眼在接受手术显微镜水平位时照度为(22 000±1 000)Lux,30 min的持续光照射.光照后继续给药1周,观察各组视网膜感光细胞中Bax、bcl-2表达差异.结果模型组较正常组bcl-2表达减弱、Bax表达增强(P<0.05),高、中、低剂量组较模型组bcl-2表达增强、Bax表达减弱(P<0.05).结论手术显微镜视网膜光损伤动物模型造模成功,复方光明胶囊各剂量组对视网膜光损伤后有保护作用,其中高、中剂量组有较为明显的优势.
Objective To observe the effect of Compound Light Capsule on the expression of Bax, bcl-2 in retina of rats with retinal light injury. Methods 30 SD rats （60 eyes） were divided into high dose group, middle dose group, low dose group, sham group, and normal group in random, 6 SD rats （12 eyes） in each group. Rats were given intragastric administration of Compound Light Capsule 7days before modeling for 2 weeks. Experimental rats in each group were exposed to the light 150 ±3mm away from the corner except the normal group. The illumination of eyes in the horizontal position of operation microscope was 22000 ±1000 lux, and light exposure lasted for 30 minutes. Rats were given Compound Light Capsule for another 1 week after exposue to the light, then the expression of Bax and bcl-2 in rats in each group were observed. Results Compared with the normal group, the expression levels of bcl-2 decreased and the expression levels of Bax increased in sham group （P 〈 0.05 ）. The expression levels of bcl-2 increased and the expression levels of Bax decreased in high, medium and low-dose group （P 〈 〈0.05）. Conclusions The establishment of the retinal light injury model is successful by the use of the operating microscope. Each dose of Compound Light Capsules shows a protective effect on retinal light injury, and high dose has the best efficacy.
出处
《昆明医科大学学报》
CAS
2012年第12期15-17,32,共4页
Journal of Kunming Medical University
基金
云南省科技厅自然科学研究基金资助项目(2009ZC153M)
云南省教育厅科研基金资助项目(09Y0237)
