摘要
目的探讨两个不同公司的同一种抗体神经-肿瘤腹侧抗原(Nova1)在大鼠脑组织中的表达,比较其异同.方法采用免疫组织化学法(IHC)和Western-blot(WB)进行检测.结果 IHC显示Nova1在大脑中分布广泛,在神经细胞的轴突或树突中都有表达;WB显示在55 kDa处都有很清晰的条带.结论这两种抗体的表达清晰,既可以用于IHC,也可以用于WB实验.
Objective To compare the expression of Neuro-oncological ventral antigen 1 （Noval, neuron-specific splicing factor） in rat brain with two antibodies from different company. Method Immunohistochemistry （IHC） and Western-blot （WB） were performed to detect the expression of Noval. Results Noval was expressed almost in the whole brain, especially in dendrites and axons. WB showed clearly bands at 55kDa. Conclusion Our data suggest that two antibodies can be used in IHC and WB experiment with same quality.
出处
《昆明医科大学学报》
CAS
2012年第12期33-35,共3页
Journal of Kunming Medical University
基金
国家自然科学基金资助项目(81100862
31100652)
