3种提取接触性检材脱落表皮细胞DNA方法的实验研究

Comparison of Three Methods for Extracting DNA from Shedding of Epidermal Cells in Contact Material

目的对3种提取接触性检材脱落表皮细胞DNA的方法进行实验研究和比较,以获得最佳的方法.方法分别采用Chelex-100小体系法、磁珠法、改良磁珠法法对脱落表皮细胞的DNA进行提取,并对检测结果进行分析比较,探讨提取接触性检材脱落表皮细胞DNA的方法.结果磁珠法和改良磁珠法均能获得较高质量的细胞核DNA STR基因座分型,且后者的分型效果更好.Chelex-100小体系法的STR基因座分型效果不佳.结论磁珠法和改良磁珠法均能有效检测接触性检材脱落细胞DNA,故在法医检案中遇到接触性检材时考虑选择改良磁珠法.

Objective To discuss the most effective DNA extracting methods from shedding epidermal cells in contact materials three by comparing methods. Method DNA was extracted from samples by the small volume of Chelex-100, Bead method and Improved Bead method. Results The STR loci genotyping result was most ef- fective by using Improved Bead method to extract DNA. The small volume of Chelex-100 can not obtain the satisfied results of STR loci genotyping. Conclusions Bead method and Improved Bead method can have a satisfied STR loci genotyping. Improved Bead method is the first choice in the actual detection of trace biological evidence.