少突胶质前体细胞修复损伤脊髓的组织学变化

Histological changes of oligodendrocyte precursor cells for repair of spinal cord injury

背景:脊髓损伤的发病率呈上升趋势,而脊髓损伤后的修复机制尚不完全清楚。目的:探讨少突胶质前体细胞在脊髓损伤修复过程中的作用。方法:采用Allen's重物撞击法建立小鼠脊髓损伤模型。病理学检测脊髓损伤程度,体外分离、纯化和诱导分化绿色荧光蛋白转基因鼠的少突胶质前体细胞并移植到脊髓损伤模型鼠体内。按照不同的治疗方式分为模型组、假手术组、治疗组和对照组。结果与结论:小鼠脊髓损伤模型建模成功率100%。培养的少突胶质前体细胞具有自我增殖能力,并且可以分化为少突胶质细胞。移植后的少突胶质前体细胞不仅可以与宿主脊髓组织较好的整合,并可迁移到损伤部位,替代损伤组织。说明外源性少突胶质前体细胞可以在脊髓损伤小鼠受损部位存活并与宿主细胞较好的整合。

BACKGROUND： The incidence of spinal cord injury shows an increasing tendency, but the repair mechanism after spinal cord injury is not fully understood. OBJECTIVE： To investigate the effect of oligodendrocyte precursor cells in repair of spinal cord injury METHODS： According to Allen＇s method, models of spinal cord injury were established in mice. The morphological change of spinal cord was detected by pathological method. Oligodendrocyte precursor cells were isolated and purified from green fluorescence protein transgenic mice in vitro, and induced to differentiate into oligodendrocytes. Furthermore, oligodendrocyte precursor cells were transplanted into mice model of spinal cord injury. The experiment was divided into four groups according to different treatment methods： model group, sham-operation group, treatment group and control group. RESULTS AND CONCLUSION： The success rate for establishing the mice model of spinal cord injury was 100%. The cultured oligodendrocyte precursor cells had the ability to self-proliferate and differentiate into oligodendrocytes. After being transplanted, oligodendrocyte precursor cells could not only integrate with the host tissue of spinal cord, but also could migrate to the injury zone and replace the damaged tissue. Motor function of mice was significantly recovered by oligodendrocyte precursor cells transplantation. Exogenous oligodendrocyte precursor cells can survive in the injury zone and integrate with the host tissue of the mice after spinal cord injury.