柚皮甙诱导骨髓间充质干细胞修复兔股骨头坏死

Naringin induces bone marrow mesenchymal stem cells to repair femoral head necrosis in rabbits

背景:自体骨髓间充质干细胞体外诱导培养后的多方向分化潜能,体内植入能否修复股骨头坏死成为临床骨科的一个研究热点。目的:柚皮甙诱导的骨髓间充质干细胞修复兔股骨头坏死头的作用。方法:柚皮甙诱导兔骨髓间充质干细胞向成骨方向分化,用自制同种异体松质骨载体负载,制备兔股骨头坏死模型,造模后第3周将12只成年新西兰大白兔随机数字表法分为2组,实验组左侧置入空白载体,对照组左侧不植入任何物品;两组右侧均置入复合有诱导后骨髓间充质干细胞的载体。结果与结论:植入后8周植入物表面有大量骨痂形成,与骨端连接紧密,少部形成连续性骨桥。12周部分充填区广布相对较成熟的骨小梁,但较正常骨小梁粗、排列不够规则,可见缺损区完全被新骨代替,提示柚皮甙诱导的骨髓间充质干细胞可修复兔股骨头坏死。

BACKGROUND： Autologous bone marrow mesenchymal stem cells exhibit multipotential differentiation after in vitro culture. There is increasing concern that whether transplantation of autologous bone marrow mesenchymal stem cells can be used for repair of femoral head necrosis. OBJECTIVE： To investigate the mechanism by which naringin induces bone marrow mesenchymal stem cells to repair femoral head necrosis in rabbits. METHODS： Rabbit bone marrow mesenchymal stem cells were induced with naringin to differentiate into osteoblasts and then loaded onto cancellous bone allograft. Femoral head necrosis rabbit models were prepared. At 3 weeks after femora head necrosis induction, 12 adult New Zealand rabbits were randomly divided into two groups. In the experimental group, blank cancellous bone allograft was implanted into the left femoral head necrosis region. Identically, nothing was implanted into the left femoral head necrosis region in the control group. The right femoral head necrosis region in each group was implanted with cancellous bone allograft loaded by naringin induced bone marrow mesenchymal stem cells. RESULTS AND CONCLUTION： At 8 weeks after implantation, a large amount of callus formed on the surface of the allograft and connected tightly with bone ends, and continuous ＂bone bridge＂ formed in some parts. At 12 weeks, relatively mature bone traboculae were observed in the filling area, which were thicker, and arranged more irregularly compared with normal bone trabeculae. Bone defect region was completely covered by the newly formed bone. These findings suggest that naringin-induced bone marrow mesenchymal stern cells can be used for repair of femoral head necrosis in rabbits.