芒果苷对大鼠成骨细胞增殖作用的影响

Effect of mangiferin on the proliferation of rat osteoblasts

背景:芒果苷以及含芒果苷植物提取物具有多方面生理和药理作用,但其是否促进成骨细胞增殖尚未见报道。目的:验证芒果苷对体外培养SD大鼠成骨细胞增殖的影响。方法:取新生的SD大鼠的颅骨,二次酶消化法培养成骨细胞。应用MTT法及细胞周期的流式细胞仪检测,观察不同浓度的芒果苷对大鼠成骨细胞毒性,不同浓度的芒果苷对体外培养成骨细胞增殖的影响。结果与结论:在一定浓度范围内,不同浓度的芒果苷均可促进成骨细胞增殖、分化,并呈现出一定的量效、时效关系。芒果苷最佳促进增殖浓度为40μmol/L。药物作用时间48,72h,芒果苷20,40,80μmol/L组均有显著促进成骨细胞增殖的作用(P<0.01或P<0.05)。芒果苷浓度20,40,80μmol/L等各组细胞S期比值均较正常细胞显著增高,以芒果苷40μmol/L浓度组增高最显著(P<0.05)。表明在一定浓度范围内,芒果苷具有促进体外成骨细胞增殖的作用。

BACKGROUND：Mangiferin and its extracts have many physiological and pharmacological effects,but whether they can promote the proliferation of osteoblasts has not been reported.OBJECTIVE：To observe the effects of mangiferin on the proliferation of osteoblasts cultured in vitro from SD rats.METHODS：The osteoblasts from the skull of neonatal SD rats were cultured by the second enzyme digestion.The MTT method and flow cytometry were used to observe the cytotoxicity proliferation of osteoblasts cultured in vitro by different concentrations of mangiferin at different time points.RESULTS AND CONCLUSION：In a certain range of concentrations,different concentrations of mangiferin could promote the proliferation and differentiation of osteoblasts in a certain dose and time dependent manner.The optimal concentration of mangiferin was 40 μmol/L to promote the proliferation of osteoblasts.When the drug action time was 48 and 72 hours,20,40 and 80 μmol/L mangiferin could significantly promote osteoblast proliferation（P〈0.01 or P〈0.05）.The S-phase fraction of osteoblasts treated with 20,40,and 80 μmol/L mangiferin was significantly increased than that of normal cells,especially those treated with 40 μmol/L mangiferin（P〈0.05）.These findings indicate that in a certain range of concentrations,mangiferin can promote the proliferation of osteoblasts in vitro.