独活寄生汤对腰椎间盘突出症兔前列腺素E2的影响

Duhuojisheng decoction effects on prostaglandin E2 in rabbit models of lumbar disc herniation

背景:独活寄生汤是中医药治疗腰椎间盘突出症的基本方,但其具体的作用机制尚不明确。目的:观察独活寄生汤对腰椎间盘突出症模型兔椎间盘局部组织及血浆前列腺素E2的影响。方法:采用造模器造模法建立腰椎间盘突出症新西兰家兔模型,于造模后1周行CT检查确定造模成功后,分别给予13.8g/kg独活寄生汤、2.3mg/kg扶他林或等量生理盐水进行灌胃,2次/d,连续灌胃2周。并以正常及假手术兔作为对照。结果与结论:①苏木精-伊红染色显示腰椎间盘突出症模型兔病变髓核及纤维环组织内可见肉芽组织长入、新生血管形成及大量炎性细胞浸润,经灌胃独活寄生汤及扶他林后,可见炎性细胞浸润明显减轻。②ELISA结果显示,腰椎间盘突出症模型兔局部软组织及外周血浆中的前列腺素E2含量明显增加,给予独活寄生汤或扶他林后其含量明显下降(P<0.01),而独活寄生汤组和扶他林组比较差异无显著性意义(P>0.05)。说明前列腺素E2在腰椎间盘突出症中起重要作用,能介导体内炎性反应;独活寄生汤能通过调控腰椎间盘突出症模型兔体内前列腺素E2的表达,从而起到治疗作用,且其在抗炎方面的作用与非类固醇类解热镇痛药扶他林相当。

BACKGROUND：Duhuojisheng decoction is a basic prescription of Chinese medicine for treatment of lumbar disc herniation,but its mechanism is yet unclear.OBJECTIVE：To study the effect of Duhuojisheng decoction on prostaglandin E2 in rabbits with lumbar disc herniation.METHODS：Models of lumbar disc herniation were established in New Zealand rabbits using molding device,and verified successfully through CT examination at 1 week after modeling.The model rabbits were randomly divided into model group（treated with normal saline）,voltaren group（treated with 2.3 mg/kg voltaren） and Duhuojisheng decoction group（treated with 2.3 mg/kg Duhuojisheng decoction）.The treatment was done twice a day,continued 2 weeks.Normal rabbits and rabbits undergoing sham operation were used as controls.nucleus pulpous and annulus fibrosus tissue visible RESULTS AND CONCLUSION：①Hematoxylin-eosin staining results showed that granulation tissue ingrowth,neovascularization and massive infiltration of inflammatory cells were visible in nucleus pulpous and annulus fibrosus tissue after successful modeling of lumbar disc herniation.However,there was a remarkable relief in inflammatory cell infiltration after intragastric administration of Duhuojisheng decoction and voltaren.②The results of enzyme linked immunosorbent assay showed that prostaglandin E2 levels in local soft tissues and peripheral plasma were increased significantly after modeling,and then decreased afte administration of Duhuojisheng decoction and voltaren（P〈0.01）.However,there was no difference in prostaglandin E2 levels after administration of Duhuojisheng decoction and voltaren（P〈0.05）.These findings indicate that prostaglandin E2 plays a critical role in lumbar disc herniation,mediating in vivo inflammatory reaction.Duhuojisheng decoction can cure lumbar disc herniation through regulation of prostaglandin E2 expression,and its anti-inflammatory mechanism is similar to nonsteroidal anti-inflammatory drugs,such as voltaren.