一种新型掺锶冻干骨材料的细胞相容性

Cellular biocompatibility of a novel strontium incorporated freeze dried bone materia

背景:前期研究已成功制得掺锶冻干骨材料。目的:评价新型掺锶冻干骨材料的细胞相容性,并与普通冻干骨材料进行比较。方法:在掺锶冻干骨与普通冻干骨材料表面直接接种MC-3T3成骨细胞,评价成骨细胞的增殖活性、碱性磷酸酶活性及骨钙素活性,并用扫描电子显微镜观察细胞生长微观形态。结果与结论:掺锶冻干骨组成骨细胞生长正常,接种第3,5天细胞数量显著高于普通冻干骨组(P<0.05),细胞碱性磷酸酶活性始终高于普通冻干骨组(P<0.05)。两组成骨细胞骨钙素水平差异无显著性意义(P>0.05)。在掺锶冻干骨材料表面的成骨细胞伸展良好,细胞呈多角形或梭形,胞浆丰富,细胞伪足末端与材料表面紧密附着,细胞突起末端可以观察到一些细小的微突触结构。表明掺锶冻干骨材料具有优良的细胞相容性,其表面成骨细胞的生长状况优于普通冻干骨材料表面的成骨细胞。

BACKGROUND：A strontium incorporated freeze dried bone material has been developed in previous report. OBJECTIVE：To evaluate the cellular biocompatibility of a novel strontium incorporated freeze dried bone material in comparison with normal material. METHODS：MC-3T3 osteoblasts were inoculated on the surface of strontium incorporated freeze dried bone material and normal freeze dried bone material, and the cell proliferation, alkaline phosphatase activity and osteocalcin activity were evaluated and cell micro morphology were observed by scanning electron microscope. RESULTS AND CONCLUSION：Osteoblast grew normally on the strontium incorporated freeze dried bone material. On days 3 and 5, cell quantity on the strontium incorporated freeze dried bone material was significantly higher than that on the normal freeze dried bone material （P 0.05）. The alkaline phosphatase activity on the strontium incorporated freeze dried bone material was significantly higher than that on the normal freeze dried bone material （P 0.05）. But there was no significant difference of osteocalcin activity between two groups （P 0.05）. On the surface of strontium incorporated freeze-dried bone material, the osteoblast spread well. Cells were polygonal or spindle-shaped with abundant cytoplasm and the pseudopodia closely attached to the material surface. Some micro filopodia could be observed at the end of cell protrusion. Strontium incorporated freeze dried bone material had excellent cell compatibility, and the growth of osteoblasts on its surface was superior to those on the surface of normal freeze dried bone material.