聚乙酸-聚乙醇酸共聚物复合同种异体细胞修复软骨缺损

Repair of articular cartilage defects by the compound of poly lactic-co-glycolic acid and allogeneic cells

背景:以自体骨髓间充质干细胞和软骨细胞作为种子细胞修复软骨缺损可达到理想效果,但存在细胞数量不足及二次创伤等问题。目的:观察同种异体骨髓间充质干细胞和软骨细胞与聚乙酸-聚乙醇酸共聚物复合修复关节软骨的可行性。方法:将15只新西兰大白兔随机分为实验组、对照组、空白组,制作关节软骨缺损模型,分别于骨缺损处植入同种异体骨髓间充质干细胞和同种异体软骨细胞与聚乙酸-聚乙醇酸共聚物复合体、自体骨髓间充质干细胞和自体软骨细胞与聚乙酸-聚乙醇酸共聚物复合体、聚乙酸-聚乙醇酸共聚物材料。结果与结论:术后12周苏木精-伊红及Masson三色染色显示,实验组软骨缺损处可见软骨细胞,呈圆形或多角形,柱状排列,软骨陷窝形成明显,可见大量细胞外基质沉积,修复组织显示出透明软骨样,与周围软骨结合好,与底层骨结合紧密;对照组与实验组无明显差别;空白组软骨缺损处可见纤维样细胞。说明同种异体骨髓间充质干细胞和软骨细胞与聚乙酸-聚乙醇酸共聚物复合可修复关节软骨缺损。

BACKGROUND：The autologous bone marrow mesenchymal stem cells and chondrocytes as seed cells can achieve the ideal effect for repairing the articular cartilage defect, but the shortage of seed cells and secondary trauma are difficult problems for researchers. OBJECTIVE：To investigate the feasibility for repairing the articular cartilage defect by co-culture of allogenic bone marrow mesenchymal stem cells and allogenic chondrocytes mixed with poly lactic-co-glycolic acid. METHODS：Fifteen New Zealand rabbits were divided into three groups, including experimental group, control group and blank group, and the articular cartilage defect models were prepared. The articular cartilage defect was filled with allogenic bone marrow mesenchymal stem cells and the complex of allogenic chondrocytes and poly lactic-co-glycolic acid, autologous bone marrow mesenchymal stem cells and the complex of autologous chondrocytes and poly lactic-co-glycolic acid, and the poly lactic-co-glycolic acid copolymer material. RESULTS AND CONCLUSION：Hematoxylin-eosin and Masson trichrome staining at 12 weeks after operation showed that the chondrocytes could be seen in the articular cartilage defect of the experimental group with round or polygonal-shaped and in a columnar arrangement. The typical cartilage lacunae were formed and a large number of extracellular matrix depositions could be seen and the repaired tissue showed a hyaline cartilage-like and integrated well with the surrounding tissues and closed well with underlying bone. There was no significant difference between the experimental group and the control group; in the blank group, the fiber-like cells could be seen in the articular cartilage defect. It is feasible to repair articular cartilage defect by co-culture of allogenic bone marrow mesenchymal stem cells and allogenic chondrocytes mixed with poly lactic-co-glycolic acid.