无心跳供体肺移植大鼠模型的建立

Establishment of a rat lung transplantation model with non-heart beating donor

背景:大量实验表明,供肺组织及肺血管功能在适当条件下可以保存,当供体心跳停止后,肺的气体交换系统可耐受热缺血60min。目的:建立一种简单有效的无心跳供体肺移植大鼠模型。方法:60只SD大鼠随机分为有心跳供体组、无心跳供体-缺血30min组和无心跳供体-缺血60min组,每组20只。有心跳供体组供体大鼠取供肺保存在4℃低钾右旋糖苷液中4h;无心跳供体-缺血30min组供体放血处死后,维持通气30min,使供肺在热缺血30min后冷保存;无心跳供体-缺血60min组方法同无心跳供体-缺血30min组,不同之处在于供肺热缺血60min。各组大鼠循环回路连接后,于1,15,30,45,60min测出气道峰压和肺静脉血氧气分压。灌注结束后检测供肺湿质量干质量比和丙二醛水平。结果与结论:有心跳供体组和无心跳供体-缺血30min组所有指标比较差异均无显著性意义(P>0.05)。各组气道峰压随时间的延长逐渐升高,但无心跳供体-缺血60min组升高程度高于有心跳供体组和无心跳供体-缺血30min组(P<0.05)。各组供肺血氧气分压均随着时间的延长而降低,无心跳供体-缺血60min组供肺血氧气分压低于有心跳供体组和无心跳供体-缺血30min组(P<0.05)。无心跳供体-缺血60min组供肺湿质量干质量比和丙二醛水平较有心跳供体组和无心跳供体-缺血30min组高(P<0.05)。结果提示大鼠无心跳供肺热缺血30min肺移植灌注模型是一种安全有效的肺移植方法。

BACKGROUND： A large number of experiments have shown that the donor lung tissue and pulmonary vascular function can be stored under appropriate conditions. When the donor heart beating stops, the pulmonary gas exchange system can be tolerant to the warm ischemia for 60 minutes. OBJECTIVE： To establish a simple and effective rat lung transplantation model with non-heart beating donor. METHODS： Sixty Sprague-Dawley rats were randomly divided into heart beating donor group, non-heart beating donor-ischemia 30 minutes group and non-heart beating donor-ischemia 60 minutes group, there were 20 rats in each group. The lungs of the donor rats in the heart beating donor group were obtained and stored in 4 ℃ low potassium dextran solution for 4 hours; rats in the non-heart beating donor-ischemia 30 minutes group were bloodletting sacrificed, and the lungs of the rats were maintained ventilation for 30 minutes, and then performed with cold preservation after hot ischemia for 30 minutes; the treatment method in the non-heart beating donor-ischemia 60 minutes group was similar to that in the heart beating donor-ischemia 30 minutes group, the difference was that the time of hot ischemia. The peak airway pressure and pulmonary venous blood oxygen partial pressure was measured at 1, 15, 30, 45 and 60 minutes after the connection of the circulation loop in each group. After perfusion, the wet weight and dry weight ratio and the malondialdehyde level were detected. RESULTS AND CONCLUSION： There was no significant difference of all the indexes between heart beating donor group and non-heart beating donor-ischemia 30 minutes group （P 0.05）. The peak airway pressure in each group was increased gradually along with the extension of time, but the peak airway pressure in non-heart beating donor-ischemia 60 minutes group was higher than that in the heart beating donor group and non-heart beating donor-ischemia 30 minutes group （P 0.05）. The pulmonary blood oxygen partial pressure in each group was gradually decreased with the extension of time, and the pulmonary blood oxygen partial pressure non-heart beating donor-ischemia 60 minutes groupwas lower than that in heart beating donor group and non-heart beating donor-ischemia 30 minutes group （P 0.05）. The wet/dry weight ratio and the malondialdehyde level in non-heart beating donor-ischemia 60 minutes group were higher than those in heart beating donor group and non-heart beating donor-ischemia 30 minutes group （P 0.05）. Non-heart beating donor-ischemia 30 minutes is a safe and effective method for lung transplantation.