环孢素干预小肠移植物中转化生长因子β1的表达

Expression of transforming growth factor-beta 1 in intestinal graft intervened by cyclosporine A

背景:小肠移植慢性排斥反应的发生是影响其长远预后的重要因素。目的:观察环孢素对于小肠移植物中转化生长因子β1表达的影响。方法:近交系F344(RT11vr)大鼠和Lewis(RT11)大鼠作为小肠移植的供体和受体,利用显微外科技术构建异系大鼠小肠移植模型,分别在建模后7,28,60d进行移植物活检,常规病理学检测,应用Real time-PCR技术检测移植物内转化生长因子β1因子mRNA转录水平,并采用免疫组织化学方法对转化生长因子β1的表达进行定位。结果与结论:在建模后7-28d应用环孢素的过程中转化生长因子β1mRNA转录水平呈现明显减低趋势;在28-60d,停用环孢素后该因子表达则明显升高。说明环孢素对于异系小肠移植中转化生长因子β1的表达有一定的抑制作用。

BACKGROUND： The chronic rejection of intestinal graft is the important factor affecting the long term prognosis. OBJECTIVE： To observe the effect of cyclosporine A on the expression of transforming growth factor-β1 in intestinal graft in rats. METHODS： Allogeneic small intestine transplantation was performed in rats by microsurgical technology with the donor of F344（RT11vr） rats and the recipient of Lewis（RT11） rats. The graft biopsy and routine pathologic examination were performed at 7, 28 and 60 days after modeling. mRNA transcription level of transforming growth factor-β1 was detected by real time-PCR. Immunohistochemical staining was also performed to detect the expression of transforming growth factor-β1. RESULTS AND CONCLUSION： The mRNA transcription level of transforming growth factor-β1 was significantly decreased during the application of cyclosporine A at 7-28 days after modeling. At 28-60 days after modeling, the mRNA transcription level was significantly increased after termination of cyclosporine A. Cyclosporine A can inhibit the expression of transforming growth factor-β1 in allogeneic small intestine transplantation.