慢性肾功能衰竭模型大鼠血清干细胞因子测定

Serum level of stem cell factor in a rat model of chronic renal failure

背景:干细胞因子是一种多功能细胞因子,其可能参与了肾间质纤维化的发生发展。目的:检测慢性肾功能衰竭大鼠模型血清干细胞因子水平,探讨其与大鼠肾间质纤维化发生发展的关系。方法:健康雄性Wistar大鼠36只,随机分为2组,模型组用腺嘌呤灌胃诱发大鼠慢性肾功能衰竭,对照组用等量生理盐水灌胃。分别于实验第4、8和12周各组随机处死6只大鼠,检测血液学及尿液学指标,ELISA检测大鼠血清干细胞因子水平,苏木精-伊红、Masson染色观察大鼠肾间质纤维化程度,计算肾间质纤维化指数,分析大鼠血清干细胞因子浓度与肾功能及肾间质纤维化指数之间的相关性。结果与结论:模型组大鼠各时期血清尿素氮、肌酐、干细胞因子水平及肾间质纤维化指数均明显高于对照组(P<0.01),且上述指标均随着灌胃天数的延长逐渐增加,血清干细胞因子浓度与尿素氮、肌酐及肾间质纤维化指数均呈明显正相关(P<0.01)。提示干细胞因子可能是慢性肾功能衰竭肾间质纤维化发生发展过程中的重要参与者。

BACKGROUND： Stem cell factor is a multifunctional cytokine, which may play an important role in the pathogenesis and progression of renal interstitial fibrosis. OBJECTIVE： To detect serum stem cell factor levels in rat models with chronic renal failure, and to study the relationship between serum stem cell factor and the pathogenesis and progression of renal interstitial fibrosis. METHODS： Thirty-six healthy male Wistar rats were randomly divided into model group （adenine lavage in order to establish the rat model with chronic renal failure） and control group （physiological saline lavage at equal volume）. Six rats from both groups were sacrificed respectively at weeks 4, 8 and 12. Some serum and urine indicators were detected. Serum stem cell factor levels were measured by enzyme linked immunosorbent assay. The extent of renal interstitial fibrosis was observed by hematoxylin-eosin staining and Masson staining, and the renal interstitial fibrosis indices were calculated. Correlation between the serum stem cell factor and renal interstitial fibrosis indices was analyzed. RESULTS AND CONCLUSION： Compared with control group, the blood urea nitrogen, serum creatinine, serum stem cell factor and renal interstitial fibrosis indices were significantly higher in model group at different time points （P 0.01）. Moreover, the above indicators were gradually increased with the extension of lavage days, the serum stem cell factor level was positively correlated with blood urea nitrogen, serum creatinine and the degree of renal interstitial fibrosis （P 0.01）. The results suggest that stem cell factor may participate in the pathogenesis and progression of renal interstitial fibrosis.