南蛇藤对人肝癌HepG2细胞侵袭能力的影响

Effects of Celastrus orbiculatus extract on the invasion in human hepatocar cinoma HepG2 cells

目的:研究南蛇藤乙酸乙酯提取物(Celastrus orbiculatus extracts,COE)对人肝癌HepG2细胞侵袭能力的影响。方法:不同浓度COE(20、40、80、160、320μg/ml)处理人肝癌HepG2细胞24 h后,用MTT法检测细胞毒作用;Transwell小室法检测细胞的侵袭能力;Western blot法检测人肝癌HepG2细胞蛋白激酶B(protein kinase B,PKB,又名Akt)、磷酸化的蛋白激酶B(p-Akt)、NF-κBp65、基质金属蛋白酶-2(matrix metalloproteinases2,MMP-2)、MMP-9蛋白的表达水平。结果:COE(20、40、80、160、320μg/ml)能显著抑制人肝癌HepG2细胞的增殖能力,呈明显的剂量依赖性;COE(20、40、80μg/ml)能降低人肝癌HepG2细胞的穿膜细胞数;COE(20、40、80、160μg/ml)下调人肝癌HepG2细胞中p-Akt、NF-κBp65、MMP-2和MMP-9蛋白表达水平。结论:COE能抑制人肝癌HepG2细胞侵袭能力,其机制可能与下调p-AKT、NF-κBp65、MMP-2和MMP-9蛋白的表达有关。

To study the effect and mechanism of Celastrus orbiculatus extracts （COE） on invasion in HepG2 cells. Methods： After HepG2 cells were treated with COE at various concentrations （20, 40, 80, 160 and 320 μg/ml） for 24 h, the cytotoxicity effect was meas- ured by using the MTT assay. The invasion ability of HepG2 cells were assayed by transwell assay. The expression levels of protein kinase B （ PKB/Akt）, phospho-protein kinase B （ p-Akt）, matrix metalloproteinases-2 （ MMP-2）, MMP-9 and NF-KBp65 in HepG2 cells were exam- ined by western blotting. Results： HepG2 cells treated with COE （20, 40, 80, 160 and 320 μg/ml） at different concentrations exhibited a dose-dependent growth inhibition. COE （20, 40, 80 μg/ml） obviously reduced the invasion potential of HepG2 cells. In addition, the ex- pression levels of p-Akt, NF-KBp65, MMP-2 and MMP-9 were dramatically suppressed by COE（20, 40, 80, 160μg/ml） in a concentra- tion-dependent manner. Conclusions： COE could significantly inhibit the invasive ability of HepG2 cells via a down-regulation of the expres- sion levels of p-Akt, NF-KBp65, MMP-2and MMP-9.