碧血胶囊对大鼠急性心肌梗死模型心功能和血流动力学的影响

Effects of Bixue Capsule on cardiac function and hemodynamics in rat myocardial infarct model

目的:观察碧血胶囊对大鼠急性心肌梗塞(AMI)后血流动力学变化及心功能的影响,探讨其预防AMI的机制。方法:结扎大鼠冠状动脉左前降支造成AMI模型,成年雄性SD大鼠50只随机分为6组:假手术组、急性心肌梗塞模型(AMI)组、通心络胶囊组、碧血胶囊高、低剂量组、地尔硫卓组。观察药物对各组大鼠心率(HR)、左心室收缩峰压均值(LVSP)、左心室舒张压均值(LVDP)、左心室收缩压最大上升速率(+dp/dtmax)、左心室舒张压最大下降速率(-dp/dtmax)、左心室开始收缩至达到室内压最大上升速率时间(t-dp/dtmax)、心律失常、血清游离脂肪酸(FFA)和天冬氨酸氨基转移酶(AST)的影响。结果:大鼠AMI后,HR、mlVSP、±dp/dtmax明显降低,LVDP,AST明显升高(P<0.05),并且随着缺血时间的延长,各指标呈逐渐恢复的趋势。与AMI组相比,碧血胶囊高(968mg/kg,结扎后120分钟)、低剂量组(484mg/kg,结扎时)可显著提高LVSP,碧血胶囊高(结扎时及结扎后120分钟时)、低剂量组(结扎后30分钟)可显著提高+dp/dtmax,碧血胶囊高(结扎时及结扎后30、120分钟时)可显著提高-dp/dtmax(P<0.05),同时碧血胶囊高、低剂量组均能降低血清AST及心律失常的发生次数(P<0.05)。结论:碧血胶囊能显著改善左室收缩和舒张功能,减轻大鼠心肌缺血后心功能的下降和降低血清AST含量,对AMI后血流动力学改变有明显的改善作用,这可能是其保护心肌梗死的机制之一。

To investigate the protected effects of Bixue capsules on cardiac function and hemodynamies in rats acute myocardial infarc- tion （AMI） model and to discuss its mechanism. Methods： Rat AMI model was constructed by ligating left anterior descending coronary ar- tery and the rats were randomly divided into shame group, acute myocardial infarct model （AMI） group and the low and high dosages of Bix- ue capsule groups, Tongxinluo capsule control group and Dierliuzhuo control group. We observed the cardiac function index such as heart rate （HR）, left ventricular systulic（ L^SP）, left ventricular diastdic blood pressure （LVDP）, maximal rising and failing rate of ventricular pres- sure（ ＋ dp/dtmax ）, the time from beginning of left ventrieular systolic to maximal rising rate of ventricular pressure （t-dp/dt）, arrhythmia and serum FFA and AST. Results： After AMI, the level of HR, LVSP and ＋ dp/dtmax were lower than those before AMI, the levels of mlVDP and AST increased significantly （ P 〈 0.05）. Along with the prolongation of ischemia all indexes tended to normal level. Compared to AMI group, Bixue capsule treated groups （high doses that is 965mg/kg , at the time of the 120 minutes after ligation and low doses treated group（ that is 484 mg/kg, at the time of ligation） can significantly increased LVSP, Bixue capsule high doses treated groups（at the time of ligation and the 120 minutes after ligation ） and low doses treated groups（ at the 30 minutes after ligation ） can significantly increased ＋ dp/ dt max , Bixue capsule high doses treated groups（ at the time of ligation and the 30,120 minutes after ligation ） can significantly increased - dp/dt max （ P 〈 0.05 ） . at the same time Bixuc capsule treated groups can significantly decreased AST in the serum and the happening of ar- rhythmia （P 〈 0.05 ）. Conclusion： By improvfia~ the compliance of ventricular wall, Bixue capsule .treated groups can mitigate the decline of cardiac function and reduce the content of AST in the serum, at the same time l it significantly improve cardiac systolic and diastolic function after AMI, which maybe is one of the mechanism of Bixue capsule treated AML