番茄少量种子脂肪酸组成含量分析方法研究

Research on Analysis Method of Fatty Acid Composition in Small Tomato Seeds

提取番茄种子粗脂肪,用KOH-甲醇溶液对脂肪酸进行甲酯化,以37种脂肪酸甲酯标品为对照,采用气相色谱分析测定其脂肪酸组成及含量,建立一种快速的少量番茄种子的脂肪酸检测方法。结果表明:番茄种子样品4 mg,采用异辛烷2 mL提取5 min,2 mol/L甲醇钠酯化5 min后即可进行脂肪酸组成含量分析,使以往需要用几个小时的工作在20 min内完成,大大缩短了分析时间。番茄种子脂肪酸组成含量分析结果不受样品量、粗脂肪提取时间和脂肪酸甲酯化时间影响。通过与传统方法(GB/T 5009.6-2003,GB/T 17376-2008 5酯交换法)比较,脂肪酸相对百分含量无明显差异,该方法准确可行。

The composition of fatty acids in rude fat which was extracted from small tomato seeds and modified to methyl esters were detected by Gas chromatography （GC）, using supelco 37 fatty acid methyl ester Mixture （37 FAME） as control. The result showed that a fast analysis method was established for detecting relative percentage content of fatty acids in a small tomato seeds. Rude fat was extracted from a 4mg sample using isooctane for 5 minutes, and it was esterified using potassium hydroxide-methanol solution （2 tool/L） for 5minutes, then the solution was injected into the GC system. Using this method, the work which usually need several hours can be completed in 20 mins now, thus lots of time can be saved. The value of fatty acid composition in tomato seeds are not affected by Sample Weight, crude fat extraction time and fatty acid esterification time. Comparing this method with traditional method （ GB/T 5009.6 - 2003, GB/T 17376 - 2008 5 ester exchange method） for detecting fatty acids composition, it was confirmed that the results got from this method was accurate and feasible.