miRNA-192在乳腺癌组织中的表达

Expression of miRNA-192 in breast cancer tissues

目的比较乳腺癌组织和癌旁正常组织中miRNA-192的表达是否有差异及其与病理类型和病变进展之间的关系。方法通过整群抽样的方法选取19例乳腺癌患者手术切除的乳腺癌组织和癌旁正常乳腺组织,采用TRIzol法提取标本组织的总RNA,利用SYBR Green实时荧光定量PCR方法对miRNA-192进行定量检测,比较乳腺癌组织和癌旁正常乳腺组织miRNA-192的表达是否有差异。结果 miRNA-192在乳腺癌组织中表达量(ΔCt值)为10.58±3.78,在正常乳腺组织的表达量(ΔCt值)为11.16±3.40,差异无统计学意义。经Spearman相关分析,乳腺癌组织中的miRNA-192表达倍数(2-ΔΔCt值)与患者年龄、肿瘤大小、淋巴结转移、妊娠次数、肿瘤分期、生产次数、孕激素分泌无相关关系,仅与雌激素分泌呈负相关(P<0.01);经逐步法线性回归分析,以miRNA-192表达倍数为因变量时,各临床资料变量均未进入回归方程。结论乳腺癌患者癌组织及癌旁正常乳腺组织中miRNA-192的表达无明显差别。

Objective To know the relationship between miRNA-192 expression in breast cancer tissues, adjacent normal tissues and the pathological types and disease progression. Methods A total of 19 cases were selected through the cluster sampling, TRIzol method was used to extract the total RNA, real-time SYBR green fluorescent quantitative polymerase chain reaction （PCR） was used for quantitative detection of miRNA-192. Results Compared with the normal tissue, no significant difference was seen in the expression of miRNA-192 in breast cancer tissue （/：50.05）, and no relationship was seen between the expression of miRNA-192 in breast cancer tissue and age, tumor size, lymph node metastasis, tumor stage, number of pregnancy, number of parturition, progesterone and estrogen secretion in stepwise linear regression. Conclusion In the present study, no significant difference was seen in miRNA-192 expression between breast cancer tissue and adjacent normal tissues, this gene may not be the tumor markers of breast cancer.