摘要
目的建立HPLC测定龙柴方及其组方药材甘草中5种指标成分含量的方法,控制该方药的质量。方法 ODS C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈和0.05%的磷酸水溶液,梯度洗脱;柱温为30℃;流速1 mL.min-1;检测波长237 nm。结果此方法可以使样品达到基线分离,阴性样品无干扰,甘草苷、异甘草苷、甘草素、甘草酸和异甘草素分别在0.156 8~1.568μg(r=0.999 7)、0.157 1~1.571μg(r=0.999 8)、0.155 8~1.558μg(r=0.999 6)、0.187 1~1.871μg(r=0.996 9)和0.158 1~1.581μg(r=0.999 5)内与各自峰面积积分值呈良好的线性关系;平均回收率分别为101.47%(RSD=1.272%)、101.09%(RSD=1.937%)、101.14%(RSD=2.388%)、100.38%(RSD=1.448%)及100.86%(RSD=1.759%)(n=5)。结论该方法分离良好、精密准确,可用于龙柴方及其组方药材甘草的质量控制。
OBJECTIVE To establish a determination method of five index components to control the quality of Glycyrrhizae Radix et Rhizoma and Longchai Decoction. METHODS An ODS Cls column (4. 6 mm × 250 mm, 5 um) was adopted; the mobile phase consisted of acetonitrile-0.05% phosphoric acid with gradient elution at the flow rate of 1 mL . min-1 ;the detection wavelength was 237 nm,and the column temperature was 30 ℃ . RESULTS The five index components achieved baseline separation, and the negative sample showed no interference. The linear ranges were 0. 156 8 - 1. 568 ug for liquiritin (r =0. 999 7), 0. 157 1 - 1. 571 ug for isoliquiritin (r = 0. 999 8), 0. 155 8 - 1. 558 ug for liquiritigenin ( r = 0. 999 6), 0. 187 1 - 1. 871 ug for glycyrrhizic ( r = 0. 996 9) and 0. 158 1 - 1.58 1 ug for isoliquiritigenin (r =0. 999 5 ), respectively. The average recoveries were 101.47% ( RSD =1.272%), 101.09% (RSD = 1.937%), 101.14% (RSD = 2. 388%), 100.38% (RSD = 1.448%) and 100.86% (RSD = 1. 759% ) , respectively ( n = 5 ). CONCLUSION This method has good resolution and high precision, and can be used for the quality control of Glycyrrhizae Radix et Rhizoma and Longehai decoction.
出处
《中国药学杂志》
CAS
CSCD
北大核心
2013年第2期139-142,共4页
Chinese Pharmaceutical Journal
基金
江苏省科技厅"科技基础实施建设计划"专项(BM2009903)
江苏高校优势学科建设工程资助项目(ysxk-2010)
关键词
龙柴方
甘草
甘草苷
异甘草苷
甘草素
甘草酸
异甘草素
Longchai decoction
Glycyrrhizae Radix et Rhizoma
liquiritin
isoliquiritin
liquiritigenin
glycyrrhizic
isoliquiritigenin