高效液相色谱法同时测定龙柴方及其组成药甘草中甘草苷等5种指标成分的含量

Simultaneous Determination of Five Index Components in Glycyrrhizae Radix et Rhizoma and Longchai Decoction by HPLC

目的建立HPLC测定龙柴方及其组方药材甘草中5种指标成分含量的方法,控制该方药的质量。方法 ODS C18色谱柱(4.6 mm×250 mm,5μm),流动相为乙腈和0.05%的磷酸水溶液,梯度洗脱;柱温为30℃;流速1 mL.min-1;检测波长237 nm。结果此方法可以使样品达到基线分离,阴性样品无干扰,甘草苷、异甘草苷、甘草素、甘草酸和异甘草素分别在0.156 8～1.568μg(r=0.999 7)、0.157 1～1.571μg(r=0.999 8)、0.155 8～1.558μg(r=0.999 6)、0.187 1～1.871μg(r=0.996 9)和0.158 1～1.581μg(r=0.999 5)内与各自峰面积积分值呈良好的线性关系;平均回收率分别为101.47%(RSD=1.272%)、101.09%(RSD=1.937%)、101.14%(RSD=2.388%)、100.38%(RSD=1.448%)及100.86%(RSD=1.759%)(n=5)。结论该方法分离良好、精密准确,可用于龙柴方及其组方药材甘草的质量控制。

OBJECTIVE To establish a determination method of five index components to control the quality of Glycyrrhizae Radix et Rhizoma and Longchai Decoction. METHODS An ODS Cls column （4. 6 mm × 250 mm, 5 um） was adopted; the mobile phase consisted of acetonitrile-0.05% phosphoric acid with gradient elution at the flow rate of 1 mL . min-1 ;the detection wavelength was 237 nm,and the column temperature was 30 ℃ . RESULTS The five index components achieved baseline separation, and the negative sample showed no interference. The linear ranges were 0. 156 8 - 1. 568 ug for liquiritin （r =0. 999 7）, 0. 157 1 - 1. 571 ug for isoliquiritin （r = 0. 999 8）, 0. 155 8 - 1. 558 ug for liquiritigenin （ r = 0. 999 6）, 0. 187 1 - 1. 871 ug for glycyrrhizic （ r = 0. 996 9） and 0. 158 1 - 1.58 1 ug for isoliquiritigenin （r =0. 999 5 ）, respectively. The average recoveries were 101.47% （ RSD =1.272%）, 101.09% （RSD = 1.937%）, 101.14% （RSD = 2. 388%）, 100.38% （RSD = 1.448%） and 100.86% （RSD = 1. 759% ） , respectively （ n = 5 ）. CONCLUSION This method has good resolution and high precision, and can be used for the quality control of Glycyrrhizae Radix et Rhizoma and Longehai decoction.