纳秒脉冲处理A375细胞裸鼠皮下移植瘤的疗效评估

Treatment Effect Assessment of A375 Cell Subcutaneous Transplantable Tumor in Nude Mouse with Nanosecond Pulsed Electric Fields

ns脉冲电场可以诱发肿瘤细胞的结构和功能发生一系列变化,使其在临床肿瘤治疗中展现出巨大的应用前景。目前,受限于检测评估手段,相关动物实验研究尚不充分。将ns脉冲(电场强度20kV/cm、脉宽200ns、重复频率5Hz、2 000个脉冲)作用于A375裸鼠皮下移植瘤,采用荧光活体成像方法定量研究ns脉冲的抑瘤效果,同时用相机随访观察局部疤痕的转归情况。与对照组荧光增加相比,处理组荧光强度逐渐减弱(检验水准P<0.05),且在第2次处理后14d荧光完全消失;随着时间的推移,治疗区域局部疤痕逐渐变浅,并在第2次处理后22d完全消失。实验结果表明,ns脉冲可以实现局部肿瘤组织的完全切除,且能实现皮肤疤痕的有效转归。

Structural and functional changes can be found in tumor cells after nanosecond pulsed electric fields（nsPEF） treatment,so nsPEF has a great application prospect in clinical tumor treatment.At present,animal experiments are not well studied due to limit of testing and assessing methods.A set of nsPEF（with field intensity of 20 kV/cm,pulse duration of 200 ns,repetitive rate of 5 Hz,and pulse number of 2 000） was performed on A375 subcutaneous transplantable tumors,then fluorescence in vivo imaging was used to trace tumor inhibition quantitatively,and time lapse healing of scars was tested by camera follow-up visit.Compared with the control group,fluorescence significantly decreased in treated group（testing level P0.05）,and no fluorescence appeared after the second nsPEF treatment for 14 days;lapse healing of scar was found after nsPEF treatment： no scar was found after the second nsPEF treatment for 22 days.The experimental results show that local tumor tissue can be completely ablated by nsPEF,with great lapse healing of scar in skin.