盐酸小檗碱对体外培养人牙周膜细胞炎性反应影响的初步分析

Preliminary analysis on the inflammatory response of cultured human periodontal ligament cells in vitro under the effect of berberine hydrochloride

目的:研究盐酸小檗碱(Ber)作用于体外培养的人牙周膜细胞(PDLCs),在脂多糖(LPS)刺激下,合成和分泌前列腺素E2(PGE2)、白细胞介素-1β(IL-1β)和白细胞介素-6(IL-6)的变化,初步推断Ber对牙周炎的治疗作用。方法:将体外培养的PDLCs根据加入LPS与否设定为NLPS和LPS两大组,再根据培养液中Ber终浓度0μg/mL、5μg/mL、10μg/mL和20μg/mL将其分为NLPS0、NLPS1、NLPS2、NLPS3以及LPS0、LPS1、LPS2和LPS3共八个组,用ELISA法检测上清液中PGE2、IL-1β和IL-6的浓度。结果:培养48h后,各组PGE2、IL-6和IL-1β有一定的表达量,NLPS各组该三者表达的差异无统计学意义(P>0.05);加入LPS后,LPS0组上清液中PGE2、IL-1β和IL-6表达明显升高,与NLPS各组之间差异具有统计学意义(P<0.05);在加入LPS的同时加入Ber后与LPS0组比较PGE2、IL-1β和IL-6表达明显下降(P<0.05);PGE2表达,LPS各组差异具有统计学意义(P<0.05),其中除LPS3以外,其他LPS组与NLPS各对应组差异具有统计学意义(P<0.05);IL-1β表达,LPS0与其他LPS组差异有统计学意义(P<0.05),而LPS1、LPS2和LPS3之间差异无统计学意义(P>0.05),且该三组与NLPS对应组之间差异无统计学意义(P>0.05);IL-6表达,随着Ber浓度升高,各LPS组表达逐渐降低(P<0.05),且与对应NLPS组差异有统计学意义(P<0.05)。结论:Ber可以抑制LPS刺激体外培养的PDLCs产生的炎性反应。

Objective：To evaluate the effect of Berberine hydrochloride （Ber） on periodontitis by detecting the changes in concentration of postaglandin E2 （ PGE2 ）, interleukin - 113 （ IL - 1β ） and interleukin - 6 （ IL - 6 ）, which were expressed by homan periodontal ligament cells （ PDLCs ） in vitro after simulated by lipepolysaceharide （LPS）. Methods：The PDLCs cultured in vitro were Divided into NLPS groups and LPS groups, then each group were further divided into four subgroups （NLPS0, NLPS1, NLPS2 and NLPS3; LPS0, LPS1, LPS2 and LPS3）, according to their different concentration （O/μg/rnL, 5μg/mL, 10/μg/mL and 20/μg/mL） of Ber added. And then detected the concentrations of PGE2, IL - 1β and IL - 6 in the subgroups by ELISA. Results：l：GE2, IL - 1β and 1L - 6 were detected in every subgroup after 48h. There was no statistically difference about the expressions of PGE2, IL- 1βand IL-6 among each NLPS greups（P 〉 0. 05 ）. Compared with NLPS groups, the expressions in LPSO increased （ P 〈 0. 05 ）. However, after adding Ber, the expressions reduced in LPS groups （ P 〈 0.05 ）. There were statistically differences in expressing PGE2 among each TPS groups{ P 〈 O. 05 ）. Except LPS3 , no significant differences were recorded among each corresponding LPS and NLPS groupslP 〈 0.05 ）. in expressing 1L - 1β, there were statistically difference between the I2cA） and the other LPS groups（ P 〈 0.05 ）. However, there was no statistically difference among LPS1, LPS2 and LPS3 groups（ P 〉 0.05） ; and no statistically difference were found among these three groups and corresponding NLPS groups, too（P 〉 0.05 ）. The expressions of IL- 6 in each LPS groups were reduced along with adding Ber （P 〈 0.05 ）and there were statistically difference among each corresponding LPS and NLPS groups（ P 〈 0.05 ）. Conclusions ： Ber can inhibit the LPS - stimulated imflammatory responses generated by PDLCs in vitro.