摘要
目的探讨脑缺血损伤ActA/Smads通路主要位点基因表达在信号转导中的作用及其机制。方法建立PC12细胞神经元氧糖剥夺(OGD)体外脑缺血模型,应用Real-time PCR技术,检测OGD3h、6h、9h、12h、16h、24h ActA及其受体ActRIIA和下游Smad3mRNA表达变化。结果 OGD3h PC12细胞ActβA、ActRIIA及Smad3 mR-NA表达均显著升高且达高峰;OGD6h ActβA mRNA表达有所降低并呈逐渐下降趋势;OGD6h ActRIIA表达开始下降,但其下降趋势弱于ActβA,在OGD9h仍高于正常对照组,OGD24h达最低点;OGD6hSmad3 mRNA表达也开始下降,但其下降趋势弱于ActRIIA,在OGD16h仍高于正常对照组,OGD24h达最低点(组间比较P<0.05)。结论ActA/Smads通路的活性随OGD时间呈动态变化,短时程OGD可能通过诱导神经元跨膜受体ActRIIA的上调激活该信号转导通路。
Objective To investigate the change of basic factors in the ActA/Smads signalling pathway during the process of ischemic brain injury. Method ActA, ActR Ⅱ A and Smad3 mRNA expression of neurons transformed from PC12 cells subjected to various OGD time were observed with Realtime PCR. Results Real-time PCR analysis show that ActA, ActR ⅡA and Smad3 gene expression increased significantly than control group and reached the peak after 3h of OGD,then decreased gradually(P 〈 0.05). Conclusion ActA/Smads signaling pathway in neurons transformed from PC12 cells was activated by short time ischemia injury( OGD 3h) ,ActR Ⅱ A may be an important regulator in activation and signal trans- duction of this pathway.
出处
《中风与神经疾病杂志》
CAS
CSCD
北大核心
2013年第1期15-17,共3页
Journal of Apoplexy and Nervous Diseases
基金
吉林省科技厅资助项目(201015181
20120723)