灵长类动物肠道沙门氏菌的分离鉴定与药敏试验

Isolation,Identification and Antibiotics Sensitivity of Intestinal Salmonella of Non-human Primates

从13个动物园的非人灵长类动物中采集了384份粪便样品,经过细菌分离培养后,并经染色镜检和生化试验等分离鉴定出153株沙门氏菌,分离率为39.84%。按照美国临床实验室标准化委员会(CLSI)的判定标准,对分离得到的153株沙门氏菌进行了22种抗生素药物的药敏试验,结果表明,153株均表现出不同的程度的耐药性,其中,羧苄西林的耐药率最高,为95.42%,而亚胺硫霉素最敏感,敏感率为90.20%;且153株沙门氏菌分离株均为多重耐药菌株(n≥2),其中1株的耐药数可达18种之多,耐2～12种抗菌药物的菌株多达123株,占分离菌株的80.39%。从不同动物园分离的沙门氏菌对22种抗生素耐药情况的方差分析来看,在所调查的13个动物园中,不同动物园沙门氏菌分离株的耐药情况也不同。

A total of 384 fecal samples were collected from 13 zoological gardens and examined for intestinal Salmonella.153 strains of Salmonella were isolated and identified by series of tests,including isolation of bacteria,biochemical tests,etc.The separation rate was 39.84%.According to the CLSI,antimicrobial susceptibility test,against 22 kinds of common antibiotics and other antibiotics,was carried out for the 153 strains of Salmonella.Our results indicated that the 153 strains of Salmonella showed varying degrees of drug resistance.They were of the highest resistance to carbenicillin（drug resistance rate,95.42%）.They had the highest sensitivity to imipenem（sensitivity rate,90.20%）.The phenomenon of multiple drug resistance occurred in all strains of Salmonella.One of these strains was resistant to 18 kinds of antibiotics.123 strains,80.39% of all strains isolated,were indentified to be resistant to 2 to 12 kinds of antibiotics.Variance analysis of resistance to 22 kinds of common antibiotics was conducted,and the results suggested that there were differences in drug resistance among the Salmonella strains collected from 13 zoological gardens.