光合产氢细菌优势菌群富集及其产氢实验研究

ENRICHMENT PREDOMINANT GROUP OF HYDROGEN-PRODUCING PHOTOSYNTHETIC BACTERIA AND THEIR HYDROGEN PRODUCTION EXPERIMENT

利用特殊培养基从光照充裕、有机质含量高的猪场粪便排放处的污泥中富集培养光合细菌混合产氢菌群,对该混合菌群的产氢培养基进行优化,并研究混合菌群的产氢特性。实验结果表明,此菌群的最佳产氢培养基配方为:氯化铵0.4g/L,氯化镁0.2g/L,酵母膏0.1g/L,磷酸氢二钾0.5g/L,氯化钠2.0g/L,谷氨酸钠3.5g/L。此菌群以1%的葡萄糖为基质时,产氢时间长达204h,最大产氢量为3.41L/L,最大产氢速率为44.17mL/(L.h),最高氢气含量为46.73%,具有工业化应用价值。

Using special culture medium, hydrogen production photosynthetic bacteria （PSB） group was enriched from the sludge of one pig farm with plentiful light and organic compounds. The optimum composition of hydrogen production culture medium for the photosynthetic bacteria was obtained through optimization research, which is am- monium chloride 0. rig/L, magnesium chloride 0.2g/L, yeast extract 0.1 g/L, potassium phosphate dibasic 0.5g/L, sodium chloride 2g/L and sodium glutamate （MSG） 3.5g/L. With glucose concentration 1% as substrate, hydro- gen-producing characters were studied as following, hydrogen production time of 204h, the maximum hydrogen pro- duction amount of 3.41 L/L, the maximal hydrogen production rate of 44.17mL/（ L· h）, the highest hydrogen con- tent percentage of 46.73%, which is valuable in industrial application.,