白藜芦醇增加脂肪细胞3T3-L1中脂联素表达的作用机制探讨

Mechanisms of Resveratrol-mediated Increasing of Adiponectin Expression Increased in 3T3-L1 Adipocytes

目的探讨白藜芦醇增加脂肪细胞3T3-L1中脂联素表达的作用机制。方法设立空白对照组、白藜芦醇组、肿瘤坏死因子(TNF-α)组及白藜芦醇+TNF-α组。用白藜芦醇、TNF-α或者二者联合处理3T3-L1脂肪细胞。用酶联免疫吸附法测定脂联素水平;实时定量PCR测定脂联素和过氧化物酶体增生物激活受体(PPAR-γ)mRNA水平;用PPAR-γ转录因子试剂盒测定PPAR-γ活性。结果与空白对照组比较,白藜芦醇组中PPAR-γ及脂联素mRNA在3T3-L1脂肪细胞中的表达增加(P<0.05);TNF-α组中脂联素mRNA表达及释放减少(P<0.05)。与TNF-α组比较,白藜芦醇+TNF-α组中脂联素mRNA的表达及分泌增加(P<0.05);白藜芦醇拮抗TNF-α对分化3T3-L1脂肪细胞中PPAR-γmRNA表达及活性抑制(P<0.05)。结论白藜芦醇能够通过调节PPAR-γ的转录活性继而拮抗TNF-α诱导下调3T3-L1脂肪细胞中脂联素的表达及分泌。

Objective To investigate the mechanism by which resveratrol enhances adiponectin expression in 3T3-L1 cells. Methods 3T3-L1 cells were treated with vehicle,resveratrol,TNF-c~,or combination of resveratrol and TNF-α;released adiponectin was measured by ELISA;adiponectin and PPAR-γ mRNA levels were analyzed using real time PCR;PPAR-γ activity was determined with PPAR-γ activity kit. Results Compared with vehicle-treated control, PPAR-T and adiponectin expressions were enhanced and suppressed in differentiated 3T3-L1 cells treated with resveratrol and TNF-α, respectively. Compared with TNF-α alone treatment, combined treatment with resveratrol re- covered TNF-α mediated suppression of adiponectin expression and release ; resveratrol compromised the suppressive effect of TNF-α on mR- NA expression and activity of PPAR-γ （P 〈 0.05）. Conclusion Resveratrol recovered TNF-α mediated reduction of adiponectin expression and release via regulation of PPAR-γ activity.