不同同剂量^137Cs γ射线照射对小鼠造血系统的影响

Effects of different 137^Csγradiation dose on mouse hematopoietic system

目的探讨不同剂量γ线照射对不同品系小鼠造血功能的影响。方法用^137Cs γ射线分别对IRM-2、ICR、615品系小鼠进行-次性全身4．0Gy γ照射，在照后不同时间检测小鼠外周血白细胞、骨髓有核细胞（BMNC）的变化。对IRM-2小鼠、C57BL／6小鼠进行6．0Gy γ射线照射，照后第45日，检测小鼠外周血象的变化。结果IRM-2、ICR、615品系小鼠经4．0Gγ照射后第2日，白细胞和BMNC数量降至最低值。第9日，IRM-2小鼠的BMNC计数与ICR、615小鼠相比，差异有统计学意义（拉3．725，P〈0．01；t=-8．487，P〈0．001）。第12日，IRM-2小鼠白细胞计数与ICR、615小鼠相比，差异有统计学意义（t=4．811和4．302，P均〈0．001）。第21日，IRM-2、ICR、615小鼠白细胞计数分别恢复到正常值的52．0％、60．7％、50．8％；BMNC计数分别恢复到正常值的90．8％、82．1％、75．4％。IRM．2、C57BL／6小鼠经6．0Gy γ射线照射后第45日，IRM-2小鼠外周血白细胞、红细胞、血红蛋白（Hgb）、红细胞压积（Hot）计数均明显高于C57BL／6小鼠（t=5．629、7．788、4．929和6．064，P均〈0．001）；IRM-2小鼠白细胞、红细胞、Hgb、Hct数量分别恢复至正常值的75．00％、98．95％、98．78％、97．55％；C57BIJ6小鼠白细胞、红细胞、Hgb、Hct数量分别恢复至正常值的40．60％、93．88％、93.31％、93．84％。结论IRM-2、ICR、615、C57BL／6小鼠受照后造血功能的恢复趋势相同，但IRM-2小鼠在辐射损伤后其造血功能的恢复较快于ICR、615、C57BL／6小鼠。

Objective To discuss the effects of different dose of radiation on the mouse＇s hemato- poietic system. Methods Mice of 615 strain, ICR strain and IRM-2 strain were given a one-time 4.0 Gy total body irradiation, and then the changes of peripheral white blood ceils and bone marrow nuclear ceils （BMNC） among those mice were observed at different time points. Mice of 1RM-2 and C57BL/6 were given a one-time 6.0 Gy total body irradiation and the changes of peripheral hematological between the two strains were observed forty-five days after irradiation. Results Two days after irradiation, white blood cell and BMNC counts decreased to the lowest level in all the mice of 615 strain, ICR strain and IRM-2 strain. During the following days, white blood cell and BMNC counts raised again. Nine days after irradiation, BMNC count in IRM-2 mice was significantly higher than that in 615 and ICR mice（t=3.725, P〈0.01; t=8.487, P〈0.001 ）. Twelve days after irradiation, white blood cell count in IRM-2 mice was significantly higher than that in the 615 and ICR mice （t=4.811 and 4.302, both P 〈0.001）. Twenty-one days after irradiation, compared to the control group, white blood cell count was recovered to 52.0%, 60.7% and 50.8% of the normal range in IRM- 2, ICR and 615 mice separately and BMNC count was recovered to 90.8%, 82,1%, 75.4% of the normal range in IRM-2, ICR and 615 mice separately. Forty-five days after 6.0 Gy irradiation, white blood cell, BMNC, hemoglobin and hematocrit counts in IRM-2 mice were significantly higher than those indexes in C57BL/6 strain（t=5.629, 7.788, 4.9289, 6.064, all P〈0.001 ）. Compared to the control group, white blood cells, red blood cells, hemoglobin and hematocrit were recovered to 75.0%, 98.9%, 98.7%, and 97.5% of the normal range separately in IRM-2 mice. And white blood cells, red blood cells, hemoglobin and hematocrit were recovered to 40.6%, 93.8%, 93.3%, and 93.8% of the normal range separately in C57BL/6 mice. Conclusion There are similar recovery trends of hematopoiesis in all strains of mice after irradiation, but IRM-2 mice recovery more quickly than the other strains of mice.