电离辐射对原代成骨细胞M-CSF表达的影响

Effects of radiation on macrophage colony stimulating factor in primary osteoblasts

目的研究辐射对于原代成骨细胞巨噬细胞集落刺激因子（M—CSF）表达的影响，进而研究辐射导致骨损伤的分子机理。方法采用原代骨髓间充质干细胞诱导分化为成骨细胞，经0、2、4Gy ^137Cs γ射线照射后，采用实时定量PCR以及Western blot方法检测辐射对M．CSFmRNA和蛋白表达水平的影响。结果2Gy和4Gyγ射线照射均能引起成骨细胞M—CSFmRNA（t=-17．329，P〈0．01；t=-3．841，P〈0．05）和蛋白表达水平上调。4Gy照射后则会导致成骨前体细胞M—CSFmRNA表达水平上调（t=-4．478，P〈0．05），但与对照组比较，两者的蛋白表达水平未见显著差异。结论2Gy和4Gy1射线照射后，成骨细胞M—CSF表达水平上调能增强核因子κB受体活化因子配体对破骨细胞分化、成熟的促进作用，有助于增强破骨细胞的骨吸收能力。

Objective To investigate the radiation effect on the expression of macrophage colony stimulating factor（M-CSF）in primary osteoblasts and the molecular mechanism of bone injury induced by radiation. Methods Osteoblasts differentiated from bone marrow stromal ceils （BMSCs）were analyzed by real-time PCR and Western blot after 2 Gy or 4 Gy 137Cs γ-irradiation. Results M-CSF mRNA and protein expression level were up-regulated after 2 Gy and 4 Gy irradiation （t=-17.329, P〈0.01; t=-3.841, P〈0.05） in osteoblasts. 4 Gy irradiation increased M-CSF mRNA in osteoblast precursors（t=-4.478, P〈0.05 ）, but do not affect the protein expression level. Conclusions These results indicated that up-regulated M-CSF in osteoblasts could enhance the function of osteoelasts＇ differentiation and maturation which induced by recep- tor activator of nuclear factor κB ligand after 2 Gy and 4 Gy irradiation. Further more, up-regulated M-CSF expression could enhance osteoclastic bone resorption of mature osteoclasts.