J亚群禽白血病病毒感染鸡的抗原和血清抗体变化规律

Variation of Antigen and Antibody in Chickens Infected with Avian Leukosis Virus Subgroup J

为了解鸡感染J亚群禽白血病病毒(ALV-J)后抗原和血清中抗体的变化规律,本研究经卵黄囊途径对6日胚龄SPF鸡胚,肌肉途径对31日龄和56日龄的SPF鸡感染ALV-J后,于不同时间采集胎粪或泄殖腔棉拭子,同时采集血液、分离血清,以检测p27抗原、J型抗体和p27抗体。结果显示,胚胎组在各检测点均可检测到p27抗原(仅1只鸡在1日龄时为阴性),而31日龄组和56日龄组检测不到p27抗原;胚胎组没有检测到p27抗体,31日龄组中80%的鸡在3次重复感染后可检测到p27抗体,56日龄组在首次感染后p27抗体的检出率较高(60%);各组J型抗体在各检测点基本为阴性(仅个别鸡在个别检测点为阳性)。结果提示,p27抗原和抗体的检测在ALV-J感染的早期诊断及禽白血病净化工作中具有重要意义。

To make clear the variation of antigen and antibody of chickens infected with avian leukosis virus subgroup J （ALV-J）,we inoculated SPF chicken embryos with ALV-J （JS09GY3） at 6th day of embryonation via yolk sac and infected SPF chickens with ALV-J （JS09GY3） at 31 and 56 days of age via intramuscular injection. Then, p27 antigen and antibodies against ALV-J at different days were detected. The results showed that p27 antigen was positive in embryo infection group during all tested time except one chicken at 1 day of age. It was interesting that p27 antigen was negative in other two groups （31-day-age embryo infection group during and 56-day-age infection groups）during all test time. p27 antibody was negative for all test time. However, p27 antibody of 80% chickens was positive in 31-day-age group after three superinfection and 60% positive chickens in 56-day-age group after first infection. ALV-J antibody of three groups at all test time was negative except individual chicken. These results suggested that detection of p27 antigen and p27 antibody would play an important role in early diagnosis of ALV-J infection and eraduation of avian leukosis in flocks.