摘要
目的研究以角鲨烯作为新型佐剂制备的新城疫、禽流感H5N1和H9灭活疫苗在SPF鸡体内的抗体效价水平以及对机体的细胞免疫作用。方法本试验油相中按一定的比例添加角鲨烯,按照常规方法制备灭活疫苗,免疫SPF鸡后,采用微量血凝抑制法和MTT法分别检测了SPF鸡免疫不同疫苗后机体的抗体效价水平和机体外周血T淋巴细胞的动态增殖变化。结果免疫以角鲨烯作为新型佐剂制备的新城疫、禽流感H5N1和H9灭活疫苗后,HI抗体在免疫后第2周检测为阳性,第3周达到最大值,持续到第4周抗体效价无明显下降(P>0.05);免疫组外周血T淋巴细胞增殖显著高于对照组。结论表明以角鲨烯作为新型佐剂制备的新城疫、禽流感H5N1和H9灭活疫苗对SPF鸡的促抗体效价作用和细胞免疫作用均有显著增强。
Objective This study evaluates the effects of squalene adjuvant on the immune responses in SPF chicken model. Methods The adjuvanticity of squalene in newcastle disease （ND）, avian influenza （AI） HSN1 and H9 was investigated by examining antibody tilers and lymphocyte proliferation follow ing immunization in chicken by micro-hemagglutination inhibition method and MTT method. Results Result shows that after given inactivated vaccine with squalene adjuvant, HI antibody was detected as positive after 2 weeks of immunization and reached maximum during the 3rd week, and there was no significant decline in the 4th week （P〉0. 05）; peripheral blood T-lymphocyte proliferation in the im munized group was significantly higher than that in the control group. Conclusion In conclusion, ND, HSN1 and H9 inactivated vaccine prepared with squalene adjuvant can increase the antibody tiler and cellular immune function in SPF chicken.
出处
《中国海洋药物》
CAS
CSCD
北大核心
2013年第1期17-22,共6页
Chinese Journal of Marine Drugs
基金
国家高技术研究发展项目(家禽病毒病基因工程疫苗的创制
2011AA10A209)资助
关键词
角鲨烯
新型佐剂
抗体效价
淋巴细胞增殖
squalene
new adjuvant
antibody tiler
lymphocyte proliferation
